抗纹枯病数量性状基因qSB-11HJX及qSB-9TQ改良粳稻品种的抗性研究  

Improvement of japonica rice resistance to sheath blight disease by incorporating quantative resistance genes qSB-11HJX and qSB-9TQ

作　　者：李明友 王嘉楠 王广达 冯志明 叶英豪 姜伟 左天 张亚芳[1,2] 陈夕军 潘学彪[1] 马玉银[3] 陈宗祥[1,2] 左示敏[1,2] LI Mingyou;WANG Jianan;WANG Guangda;FENG Zhiming;YE Yinghao;JIANG Wei;ZUO Tian;ZHANG Yafang;CHEN Xijun;PAN Xuebiao;MA Yuyin;CHEN Zongxiang;ZUO Shimin(Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,College of Agriculture,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China;Yangzhou Polytechnic Colleg,Yangzhou 225009,China)

机构地区：[1]扬州大学农学院/江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室,江苏扬州225009 [2]扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009 [3]扬州市职业大学,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2019年第6期1-7,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31571748);江苏省自然科学基金资助项目(BK20171293);江苏省重点研发计划项目(BE2018351-1);扬州市职业大学2018年度校级重点科研项目(yzpc2018014);江苏省大学生创新创业训练计划项目(201711117054Y);植物功能基因组学教育部重点实验室开放课题(ML201806);江苏高校优势学科建设工程项目(2014-12)

摘　　要：利用分子标记辅助选择,将华粳籼74中抗纹枯病数量性状基因qSB-11HJX回交导入不同来源的粳稻品种中,并在连粳6号和南粳9108背景下,将其与来自特青的抗纹枯病数量性状基因qSB-9TQ进行聚合。对BC1F2群体、BC1F3株系以及BC3F3以上世代株系进行人工接种鉴定。结果表明:同一背景不同世代材料中,携带qSB-11HJX株系的纹枯病病级均显著低于轮回亲本,表明这些粳稻品种在qSB-11HJX相应座位上均携带感病等位基因,qSB-11HJX可用于这些粳稻品种的纹枯病抗性改良;BC4F3鉴定结果表明,在0~9级病级评价标准下,qSB-11HJX平均可减轻病级0.78~1.22级。对2个背景中的qSB-11HJX和qSB-9TQ聚合株系进行接种鉴定,结果显示,在同一背景下携带qSB-11HJX或qSB-9TQ的单基因系,其病级均显著低于轮回亲本,但均显著高于双基因聚合系,表明qSB-11HJX和qSB-9TQ的聚合有利于进一步提高纹枯病抗性。The resistant allele(qSB-11HJX) of quantitative trait locus(QTL) qSB-11 on Huajingxian 74(HJX74) was employed to improve sheath blight(SB) resistance of japonica rice varieties from different ecological regions by backcross and molecular marker-assisted selection. In the background of Lianjing 6 and Nanjing 9108, the qSB-11HJX and the other quantitative resistance gene qSB-9TQ from rice variety Teqing were pyramided. The rice lines, include BC1F2bulked populations, BC1F3 lines, and some advanced backcross lines, were artificially inoculated with SB fungus in field. Results showed that the plants carrying qSB-11HJX from different generations under the same background were all significantly more resistent to SB than those without qSB-11HJX, indicating these japonica rice varieties carried susceptible allele(s) in qSB-11 loci and qSB-11HJX could be used to improve japonicarice SB resistance.By using BC4F3 lines,we evaluated that qSB-11HJX could reduce SB disease scores by 0.78-1.22 under different genetic backgrounds in‘0-9’SB disease scoring standard.Further,we found that in each of the backgrounds,Lianjing 6 and Nanjing 9108,the SB disease scores of lines carrying single gene were all significantly lower than those without the two genes but all significantly higher than those with the both genes.This indicates that pyramiding qSB-11HJX and qSB-9TQ is able to further improve SB resistance of japonicarice varieties.

关 键 词：粳稻 纹枯病 抗性QTL 基因聚合 抗性改良 

分 类 号：S511[农业科学—作物学]