灵芝连作土壤真菌群落分析  

Analysis of fungal community in continuous cropping soil of Ganoderma lingzhi

作　　者：袁源 黄海辰 叶丽云 傅俊生[1] 吴小平 YUAN Yuan;HUANG Hai-Chen;YE Li-Yun;FU Jun-Sheng;WU Xiao-Ping(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《菌物学报》2019年第12期2112-2121,共10页Mycosystema

基　　金：食用菌资源开发和高效加工关键技术研究(2018YFD0400200)~~

摘　　要：本研究通过Illumina MiSeq平台对邻近野生土壤(GL0)、种植1年(GL1)、2年(GL2)和4年(GL4)的灵芝覆土进行ITS1扩增子测序,分析灵芝连作覆土中真菌群落的变化,为揭示灵芝连作障碍的机理提供理论依据。研究结果表明,12个灵芝土壤样本一共获得349642条有效序列,经聚类得到2426个OTU,分别隶属于8门、28纲、64目、127科、233属和449种。在门水平上,随着连作年限的增加,灵芝覆土中真菌的多样性水平逐渐减低,在GL4组中只含有担子菌门(Basidiomycota,占85.03%)、子囊菌门(Ascomycota,占14.77%)和少量被孢霉门(Mortierellomycota,占0.20%)。其中,担子菌门的相对丰度随着连作年限的增加显著增加,子囊菌门的相对丰度显著减少,而被孢霉门的相对丰度无显著性差异。在属水平上,担子菌门灵芝属Ganoderma的相对丰度随着连作年限的增加而极显著增加,子囊菌门仅青霉属Penicillium菌群的相对丰度呈现先减少后增加的趋势,在GL4组中相对于GL0组其相对丰度增加了57.92%,表明青霉属可能是引发该地区灵芝连作障碍的重要菌群,该研究为探索灵芝连作障碍的分子机制和生态防控提供了理论依据。ITS1 amplicon sequencing of the soil fungi in uncropped wild soil(GL0)adjacent to cropping soil,and 1 year cropping soil(GL1),2 year cropping soil(GL2),and 4 year cropping soil(GL4)of Ganoderma lingzhi was performed by Illumina MiSeq platform to analyze the changes of fungus communities in the continuous cropping soil,for the purpose of exploring the possible obstacle of G.lingzhi continuous cropping.A total of 349642 valid sequences was obtained from 12 G.lingzhi cropping soil samples,and 2426 OTUs was obtained by clustering,affiliated to 8 phyla,28 classes,64 orders,127 families,233 genera and 449 species.At the level of phylum,with the increase of continuous cropping years,the diversity of fungi in G.lingzhi cropping soil is gradually reduced.In GL4 experimental group,Basidiomycota,Ascomycota and Mortierellomycota are dominant,accounting for 85.03%,14.77%and 0.20%of the total taxa respectively.The relative abundance of basidiomycetes increased significantly with the increase of continuous cropping years,while that of ascomycotes significantly reduced.There was no significant difference in the relative abundance of Mortierellomycota.At the genus level,the relative abundance of Ganoderma increased significantly with the increase of continuous cropping years.The relative abundance of Penicillium in ascomycetes decreased in the beginning and then increased,whose relative abundance in GL4 increased by 57.92%as compared with that in GL0,indicating Penicillium may be a key factor obstructing continuous cultivation of G.lingzhi.

关 键 词：灵芝 真菌群落 扩增子测序 连作障碍 

分 类 号：S567.31[农业科学—中草药栽培]