金针菇单孢菌株菌丝生长速度QTL定位分析  

Growth-rate related QTL analysis of Flammulina filiformis monokaryotic isolates

作　　者：韩星 李依宁 赵琛 刘媛媛 杜慕云[1] 黄千慧 徐伟南 谢宝贵[1] HAN Xing;LI Yi-Ning;ZHAO Chen;LIU Yuan-Yuan;DU Mu-Yun;HUANG Qian-Hui;XU Wei-Nan;XIE Bao-Gui(Mycological Research Center of Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China;Horticulture College of Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学菌物研究中心,福建福州350002 [2]福建农林大学园艺学院,福建福州350002

出　　处：《菌物学报》2019年第12期2241-2248,共8页Mycosystema

基　　金：福建省种业工程专项木生型食用菌品种选育与产业化工程(fjzycxny2017010);国家食用菌良种联合攻关项目(111821301354052291)~~

摘　　要：本研究以已经完成基因组测序的单核菌株"6-3"与"6-21"为出发菌株,配对后获得有锁状联合的异核菌株并进行出菇,收集担孢子,单孢分离获得90个菌株构成作图群体,对作图群体的每个菌株进行二代测序并测定菌丝在PDA培养基的生长速度。分析"6-3"与"6-21"两单核菌株的SNP,获得68914个高质量SNP标记用于遗传连锁群分析,构建了14个遗传连锁群,总长度744.32cM,平均长度为53.17cM,标记间平均遗传距离为1.88cM。QTL分析获得一个控制菌丝生长速度的基因座qMGRP1-LG7,该基因座包含134个基因,富集了与物质代谢有关的通路和基因。The genome-sequenced Flammualia filiformis monokaryon strains"6-3"and"6-21"were used as parental strains,and the paired hybrid was cultivated to produce fruiting body.90 strains obtained from individual basidospores were constituted as mapping group.Genome sequencing of each strain in the mapping group was performed by NGS and the mycelial growth rate on PDA plates was determined.The analysis of SNPs of both the parental strains resulted in 68914 SNPs with high quality for constructing 14 genetic linkage groups.The total length,the average length and the average genetic distance between genetic markers were 744.32 cM,53.17 c M and 1.88 cM,respectively.QTL analysis showed a locus qMGRP1-LG7 controlling the mycelium growth rate,which contained 134 genes,enriching in pathways and genes related to metabolism.

关 键 词：单核苷酸多态性 遗传连锁群 数量性状基因座定位 

分 类 号：S646.15[农业科学—蔬菜学]