肝细胞癌组织CC趋化因子配体23(CCL23)表达的生物信息学分析及意义  

Bioinformatics analysis and significance of expression of CC chemokine ligand 23(CCL23)in hepatocellular carcinoma

作　　者：陆进[1,2] 杨月[3] 俞鹏[1,2] 陶恒[1,2] 卢兴浩[1,2] 王黎源 刘冬播[1,2] 陈云帆 陈传好[1,2] LU Jin;YANG Yue;YU Peng;TAO Heng;LU Xinghao;WANG Liyuan;LIU Dongbo;CHEN Yunfan;CHEN Ghuanhao(Department of Human Anatomy,Bengbu Medical College,Bengbu 233030,China;Key Laboratory of Tissue Transplantation of Anhui Province,Bengbu Medical College,Bengbu 233030,China;First Affiliated Hospital,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院人体解剖学教研室,安徽蚌埠233030 [2]蚌埠医学院组织移植安徽省重点实验室,安徽蚌埠233030 [3]蚌埠医学院第一附属医院,安徽蚌埠233030

出　　处：《细胞与分子免疫学杂志》2019年第10期903-909,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省教育厅自然科学基金重点项目(KJ2019A0338)。

摘　　要：目的探讨CC趋化因子配体23(CCL23)在肝细胞癌(HCC)组织中的表达及其对患者生存预后的临床意义.方法分别利用GEPIA、HCCDB、MetaScape、TOMER、TISIDB,Kaplan-Meier Plotter等在线数据库分析CCL23在HCC组织的表达水平、表达基因及其基因本体(GO)功能注释和京都基因与基因组百科全书(KEGG)通路富集、肿瘤细胞纯度的相关性、免疫细胞中的表达情况和对患者生存预后意义.结果CCL23在HCC各期均低表达,并且与HCC肿瘤细胞纯度负相关,低表达CCL23的HCC患者的生存期短预后差.CCL23在HCC中共表达基因的GO功能和KEGG通路主要富集在免疫细胞活化和补体系统激活等.CCL23是HCC中最强的趋化因子,可与CC趋化因子受体1(CCR1)、CCR2、CCR7和CXC趋化因子受体6(CXCR6)等多种受体结合而发挥对免疫细胞的趋化作用,其中对效应CD8^+T细胞和巨噬细胞趋化作用最明显.结论HCC组织CCL23低表达,不利于HCC患者抗肿瘤免疫防御作用的发挥,显著缩短HCC患者的生存期.Objective To investigate the expressio n of C-C motif chemokine ligand 23(CCL23)in hepatocellular carcinoma(HCC)and its clinical significance for survival and prognosis.Methods GEPIA,HCCDB,MetaScape,TIMER,TISIDB,Kaplan-Meier Plotter and other online databases were used to analyze the expression level of CCL23 in HCC,the functional notes of co-expression gene and its gene ontology(GO),the enrichment of Kyoto gene and genome encyclopedia(KEGG),the correlation between tumor cell purity,the expression of CCL23 in immune cells and its significance for survival and prog nosis of patients.Results The expressi on of CCL23 in all stages of HCC was negatively correlated with the purity of HCC tumor cells.The short prognosis of HCC patients with low expression of CCL23 was poor.The GO function and KEGG pathway of CCL23 co-expressed gene in HCC were mainly enriched in immune cell activation and complement system activation.CCL23 was the strongest chemokine factor in HCC,and it could bind to multiple receptors including CC chemokine receptor 1(CCR1),CCR2,CCR7 and CXC chemokine receptor 6(CXCR6)to exert chemokine effect on immune cells,among which CD8+T cells and macrophages have the most obvious chemokine effect.Conclusion The low expression of CCL23 in HCC tissue is not conducive to the development of anti-tumor immune defense in HCC patients and significantly shortens the survival of HCC patients.

关 键 词：肝细胞癌(HCC) 趋化因子 肿瘤 受体 配体 

分 类 号：R392.11[医药卫生—免疫学] R735.7[医药卫生—基础医学]