抗人血红蛋白胶体金免疫层析试纸条的研制  

Preparation of anti-human hemoglobin colloidal gold immunochromatography test strip

作　　者：唐嘉 袁超璐 乐美君 杜佳南 王加斌 龚海平 袁军岳 郑平安 TANG Jia;YUAN Chaolu;LE Meijun;DU Jianan;WANG Jiabin;GONG Haiping;YUAN Junyue;ZHENG Ping'an(Zhoushan Hailisheng Marine Biological Research Institute Co.,Ltd,Zhoushan 316000,China;Hailisheng Group Co.,Ltd,Zhoushan 316000,China)

机构地区：[1]舟山海力生海洋生物研究院有限公司,浙江舟山316000 [2]浙江海力生生物科技股份有限公司,浙江舟山316000

出　　处：《细胞与分子免疫学杂志》2019年第9期789-793,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的研制用胶体金免疫层析法定性检测人体粪便中血红蛋白(Hb)的试纸条。方法试纸条的玻璃纤维膜上包被胶体金标记的小鼠抗人Hb SPR-5单克隆抗体,硝酸纤维素膜的检测线(T)与质控线(C)处分别包被小鼠抗人Hb SP-5单克隆抗体和山羊抗小鼠IgG,通过双抗体夹心技术和免疫层析作用,使阳性样本在T线和C线处均显色,而阴性样本只在C线显色。结果制备的试纸条对人Hb的检测下限为21 ng/mL,与鸡、兔、羊、猪、牛这5种动物血样不发生交叉反应。结论制备的检测试纸条能明显提高人体粪便潜血的检岀率并避免假阳性结果。Objective To develop the colloidal gold immuno chromatography test strip for qualitatively detecti ng the hemoglobin(Hb)in human feces.Methods Mouse anti-human Hb monoclonal antibody SPR-5 marked by colloidal gold was coated in glass fiber membrane,and then the mouse anti-human Hb SP-5 monoclonal antibody and goat anti-mouse IgG were immobilized in testing(T)line and control(C)line located in nitrocellulose membranes,respectively.With this double antibody sandwich technique and immunochromatography test,the Hb antigen would react with both antibodies coated in the T line and C line and cause two colour reactions if the detected sample was positive,whereas the antigervantibody combination and colour reaction only showed up in the C line when the sample was negative.Results The minimum detection limit of this test strip for human Hb was 21 ng/mL and no cross reactions were found in chick Hb,rabbit Hb,sheep Hb,pig Hb and cow Hb.Conclusion The test strips can improve the detection rate of fecal occult blood obviously and avoid false-positive results.

关 键 词：血红蛋白 胶体金 免疫层析 

分 类 号：R331.1+41[医药卫生—人体生理学] R446.4[医药卫生—基础医学]