2014-2017年自贡市HIV-1抗体确证检测结果分析  

Analysis on HIV-1 Antibody Confirmation Test Results in Zigong From 2014 to 2017

作　　者：张英[1] 万晓宇 何晓冬[1] 叶黎[2] 江超 ZHANG Ying;WAN Xiaoyu;HE Xiaodong;YE Li;JIANG Chao(Zigong Center for Disease Control and Prevention,Zigong 643000,Sichuan Province,China;Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China;Gongjing Center for Disease Control and Prevention in Zigong,Zigong 643000,Sichuan Province,China)

机构地区：[1]自贡市疾病预防控制中心,四川自贡643000 [2]四川省疾病预防控制中心,成都610041 [3]自贡市贡井区疾病预防控制中心,四川自贡643020

出　　处：《预防医学情报杂志》2020年第2期219-223,共5页Journal of Preventive Medicine Information

摘　　要：目的通过对2014-2017年自贡市人类免疫缺陷病毒Ⅰ型(HIV-1)抗体初筛试验阳性样本的确证检测结果进行回顾性统计分析,为本地区艾滋病检测工作提供技术支持。方法收集自贡市23家初筛实验室筛查阳性上送确证实验的样本,使用快速检测(RT)复核,同时用免疫印迹法(WB)进行确证,应用SPSS18.0软件对2014-2017年全市确证情况进行统计学分析,率的比较采用χ^2检验。结果3413份待确证样本中,HIV抗体阳性有2806例(2806/3413)占82.2%;阴性有341例(341/3413)占10.0%;不确定有266例(266/3413)占7.8%。2806例确证阳性样本中,女性830例,男性1976例,比例为(1∶2.38),年龄以>50岁为主,占86.0%;职业以农民为主(85.4%);婚姻状况以离异为主(90.6%);文化程度以高中及以上为主(88.6%)。确证阳性带型共分为9种,基因组env带的阳性率最高,其中gp160、gp120的阳性率占100%,其次基因组gag带p24阳性率占93.3%,基因组gap带p55的阳性率最低(41.5%)。不同送检机构确证阳性结果分布差异有统计学意义(χ^2=334.762,P<0.05)。疾控机构的阳性检出率最高(880/951)92.5%。结论针对自贡市HIV检测情况,应加大艾滋病网络实验室规范化管理,严控检测质量,为尽早发现感染者提供质量保证。Objective To conduct a retrospective statistical analysis on the confirmation test results of positive samples of Human Immunodeficiency Virus-1 type(HIV-1)antibody screening in Zigong from 2014 to 2017,so as to provide the technical support for HIV testing in local district.Methods The test samples were collected from 23 screening laboratories in Zigong city for screening.Rapid test(RT)was used for re-examination,and western blotting method(WB)was used for confirmation.Statistical analysis was carried out on the confirmation status of the city from 2014 to 2017 by using SPSS18.0 software.The comparison of the rates was performed by χ^2 test.Results Among the 3413 samples to be confirmed,2806(2806/3413)were HIV antibody positive(82.2%),and 341 cases(341/3413)were negative(10.0%).There were 266 cases(266/3413)of HIV antibody uncertainty(7.8%).Among the 2806 positive samples,male were 1976 and female were 830 with the gender ratio of 2.38∶1;the aged>50 years old mainly accounted for 86.0%,the farmers took 85.4%,the divorced were 90.6%,and the high school and above accounted for 88.6%.There were 9 confirmed positive band types,and the positive rate of genomic env band was the highest,of which gp160 and gp120 accounted for 100%,followed by the positive rate of gag band p24(93.3%),and the positive rate of genomic gap band p55(41.5%).There was a statistically significant difference in the distribution of positive results among different inspection institutions(χ^2=334.762,P<0.05).The positive detection rate of CDC was the highest(880/951)92.5%.Conclusion Given the HIV testing results in Zigong,it is necessary to strengthen the standardized management of AIDS network laboratory,strictly control the quality of testing,and provide quality assurance for the early detection of infected people.

关 键 词：HIV-1抗体 免疫印迹 

分 类 号：R512.91[医药卫生—内科学]