氧化苦参碱对神经痛小鼠坐骨神经功能的修复作用研究  

Protective Effect of Oxymatrine on Sciatic Nerve Function in CCI Mice

作　　者：李黎丽 闫宇辉[2] LI Li-li;YAN Yu-hui(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110032,China;College of Medicine,Liaoning University of Traditional Chinese Medicine,Dalian Liaoning 116600,China)

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中医药导报》2019年第23期16-19,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨氧化苦参碱(oxymatrine,OMT)对神经性疼痛小鼠坐骨神经功能的修护作用。方法:将C57BL/6小鼠分为假手术组、模型组(慢性坐骨神经结扎)和氧化苦参碱组;氧化苦参碱组小鼠腹腔注射OMT,150 mg/kg,给药7 d,假手术组和模型组小鼠腹腔注射生理盐水;Von Frey纤毛笔检测各组小鼠的机械缩足反射阈值;计算坐骨神经功能指数并测定其传导速度观测坐骨神经功能恢复情况;qRT-PCR法检测各组小鼠坐骨神经IL-1βmRNA、IL-6 mRNA和TNF-αmRNA的表达;ELISA法检测各组小鼠坐骨神经IL-1β、IL-6和TNF-α炎性因子的含量。结果:氧化苦参碱组小鼠机械缩足反射阈值、坐骨神经功能指数及其传导速度均明显高于模型组;氧化苦参碱组小鼠坐骨神经IL-1βmRNA、IL-6 mRNA和TNF-αmRNA表达及蛋白含量均显著低于模型组。结论:氧化苦参碱对慢性坐骨神经结扎小鼠坐骨神经的功能损伤有一定的修护作用,其作用机制可能为减轻炎症反应所致坐骨神经损伤。Objective: To investigate the protective effect of oxymatrine(OMT) on sciatic nerve function in neuropathic pain mice. Methods: C57 BL/6 mice were randomly divided into Sham group, model group(Chronic constrictive injury group) and OMT group [CCI mice were treated with OMT(150 mg/kg) for 7 d after CCI]. The paw withdrawal mechanical threshold(PWMT) was detected by Von Frey;The sciatic nerve function was observed by calculating sciatic nerve function index and measuring its conduction velocity;The expression of IL-1β mRNA, IL-6 mRNA and TNF-α mRNA were observed by qRT-PCR;The IL-1β, IL-6 and TNF-α concentration was observed by ELISA. Results: The OMT group showed higher PWMT, sciatic nerve function index and conduction velocity than model group;The OMT group showed lower mRNA expression and protein concentration of IL-1β,IL-6 and TNF-α than model group. Conclusion: OMT shows therapeutic effect on sciatic nerve function in CCI mice, and the mechanism may be reducing damage from inflammation reaction.

关 键 词：氧化苦参碱 神经性疼痛 坐骨神经功能指数 坐骨神经传导速度 炎性反应 小鼠 

分 类 号：R285.5[医药卫生—中药学]