VEGF/PTEN及下游信号通路在侵袭性垂体瘤中的表达及其临床意义  

Expressions of VEGF,PTEN,and downstream signaling pathways in invasive pituitary adenomas

作　　者：毛建辉[1] 孙昭胜[1] 郭洪[1] 魏建辉 司娜[1] 邱雷[2] 郭连峰[3] 郑磊[4] MAO Jianhui;SUN Zhaosheng;GUO Hong;WEI Jianhui;SI Na;QIU Lei;GUO Lianfeng;ZHENG Lei(Department of Neurosurgery,Harrison International Peace Hospital,Hengshui 053000,China;Department of Pathology,Harrison International Peace Hospital,Hengshui 053000,China;Department of Clinical Laboratory,Harrison International Peace Hospital,Hengshui 053000,China;Central Laboratory,Harrison International Peace Hospital,Hengshui 053000,China)

机构地区：[1]哈励逊国际和平医院神经外科,衡水053000 [2]哈励逊国际和平医院病理科,衡水053000 [3]哈励逊国际和平医院检验科,衡水053000 [4]哈励逊国际和平医院中心实验室,衡水053000

出　　处：《武警医学》2019年第10期866-869,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨侵袭性垂体瘤中血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)与人第10号染色体缺失的磷酸酶(gene of phosphate and tension homology deleted on chromsome ten,PTEN)表达水平及其下游信号通路的变化的临床意义。方法收集医院神经外科手术后垂体瘤标本62例(侵袭组32例,非侵袭组30例)和60例正常垂体组织标本(对照组)。通过实时定量PCR和蛋白质印迹法检测比较VEGF、PTEN、磷酸化丝氨酸/苏氨酸激酶(phosphate-Serine/threonine Kinase,p-Akt)、磷酸化丝裂原活化蛋白激酶(phosphate-mitogen-activated protein kinase,p-MAPK)在垂体瘤和正常垂体组织之间的表达差异。结果垂体瘤标本中VEGF的表达水平显著高于对照组(P<0.05),在侵袭组(3.61±0.16)中的表达高于非侵袭组(2.06±0.13),差异有统计学意义(t=10.60,P<0.05),而PTEN在垂体瘤标本中的表达显著低于对照组(P<0.05),尤其在侵袭组(0.42±0.10)中的表达显著降低(t=6.31,P<0.05)。垂体瘤标本中p-Akt和p-MAPK水平显著高于对照组(P<0.05),且侵袭组(p-Akt 3.90±0.14;p-MAPK 2.52±0.11)明显高于非侵袭组(p-Akt 2.27±0.11;p-MAPK 1.89±0.05)(p-Akt,t=12.95,P<0.05;p-MAPK t=7.29,P<0.05)。结论侵袭性垂体瘤中VEGF、p-Akt及p-MAPK水平明显增高,而PTEN表达水平显著降低,提示上述变化可能与垂体瘤的侵袭性相关,其可能为诊断并治疗侵袭性垂体瘤靶点的选择提供新方向。Objective To analyze the expressions of vascular endothelial growth factor(VEGF),gene of phosphate and tension homology deleted on chromosome ten(PTEN),and downstream signaling pathways in invasive pituitary adenomas.Methods Sixty-two cases of pituitary adenomas(32 invasive and 30 non-invasive tumors)and 60 cases of normal pituitary glands were selected.The expressions of PTEN/VEGF,their downstream phosphate-serine/threonine kinase(p-Akt)and phosphate-mitogen-activated protein kinase(p-MAPK)signaling pathways in all the pituitary adenomas and normal pituitary glands were detected by quantitative polymerase chain reaction and Western blot.Results(1)There was a significant decrease of PTEN and notable increase of VEGF in patients with pituitary adenomas compared to the normal controls(P<0.05).Meanwhile,the expression levels of PTEN(0.42±0.10)and VEGF(3.61±0.16)in the invasive pituitary adenomas were significantly different from those in the non-invasive pituitary adenomas(PTEN0.86±0.11,VEGF 2.06±0.13)(PTEN t=6.31,P<0.05;VEGF t=10.60,P<0.05).(2)Levels of p-Akt and p-MAPK in the pituitary adenoma group were notably increased compared with the control group(P<0.05),whereas the invasive pituitary adenomas(p-Akt 3.90±0.14;p-MAPK 2.52±0.11)showed a statistically significant increase compared with the non-invasive pituitary adenomas(p-Akt 2.27±0.11;p-MAPK 1.89±0.05)(p-Akt,t=12.95,P<0.05;p-MAPK t=7.29,P<0.05).Conclusions Our study has offered evidence that there is a significant decrease of PTEN and notable increase of VEGF,p-Akt and p-MAPK in invasive pituitary adenomas,suggesting that these changes may be related to the invasiveness of pituitary adenomas.These findings can facilitate the diagnosis and treatment of invasive pituitary adenomas.

关 键 词：侵袭性垂体瘤 非侵袭性垂体瘤 第10号染色体缺失的磷酸酶 血管内皮细胞生长因子 磷酸化丝氨酸/苏氨酸激酶 磷酸化丝裂原活化蛋白激酶 

分 类 号：R651.1[医药卫生—外科学]