葛根及其配伍对EAMG大鼠骨骼肌病理形态及骨骼肌线粒体ATP酶的影响  

Effect of Pueraria Lobata and Its Compatibility on Pathological Morphology of Skeletal Muscle and Mitochondrial ATPase of Skeletal Muscle in EAMG Rats

作　　者：李静[1] 文颖娟[1] 杨俊超[1] 王江[1] 柏鲁宁 LI Jing;WEN Yingjuan;YANG Junchao;无(Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712046,China;The Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712000,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《四川中医》2020年第1期51-54,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金项目资助(编号:81202642);第四批全国中医(临床、基础)优秀人才研修项目(编号:国中医人教发[2017]24号);陕西省科学技术厅资助项目(编号:2017SF-323)

摘　　要：目的:探索葛根及其配伍对骨骼肌病理形态及其线粒体ATP酶的影响。方法:将160只健康大鼠随机平均分为8组,分别为正常组、模型组、强的松组、葛根组、葛芪组、葛参组、葛妙组和葛芪参妙组,用鼠源性乙酰胆碱受体97~116肽段作为免疫原,多点皮下注射以造模,成模后分别使用强的松和各组中药进行连续灌胃4周,然后取材,分别观察大鼠治疗前后骨骼肌病理形态的改变及其骨骼肌线粒体ATP酶的变化。结果:相比正常组,模型组重症肌无力大鼠骨骼肌线粒体的ATP酶活性明显降低,其Na^+-K^+-ATPase,Mg^2+-ATPase,Ca^2+-ATPase,Mg^2+-Ca^2+-ATPase活性明显低于正常组大鼠(P<0.05);治疗后,强的松组和各中药治疗组大鼠的ATP酶活性均有所恢复(P<0.05),以葛芪参妙组的恢复最为明显(P<0.01)。结论:葛根及其配伍组合可能通过提高骨骼肌线粒体ATP酶活性以改善骨骼肌病理形态,从而治疗重症肌无力。Objective:To explore the effect of Pueraria lobata and its compatibility on skeletal muscle pathology and mitochondrial ATPase.Methods:160 healthy rats were randomly divided into 8 groups:normal group,model group,prednisone group,Gegen group,Geqi group,Geshen group,Gemiao group,Geqi Shenmiao group,Rat-derived acetylcholine receptor 97~116 peptide segment was used as immunogen,and multi-point subcutaneous injection was used to establish the model.Results:Compared with the normal group,the ATPase activity of skeletal muscle mitochondria in the model group was significantly lower,and the activities of Na^+-K^+-ATPase,Mg^2+-ATPase,Ca^2+-ATPase,Mg^2+-Ca^2+-ATPase were significantly lower than those in the normal group(P<0.05);after treatment,the ATPase activity of the prednisone group and the rats in the treatment group of Chinese herbs all recovered(P<0.05),especially in the Geqi Shenmiao group(P<0.05).Conclusion:Gegen and its compatible group may improve the pathological morphology of skeletal muscle by improving the activity of mitochondrial ATPase in skeletal muscle,thus treating myasthenia gravis.

关 键 词：重症肌无力 线粒体 葛根 

分 类 号：R285.5[医药卫生—中药学]