2016-2018年北京市朝阳区流感样病例中肠道病毒病原谱构成及分子流行病学特征  

The pathogen spectrum and molecular epidemiological characteristics of enterovirus in cases of influenzalike illness in Chaoyang,Beijing from 2016—2018

作　　者：梁海旭 王海滨[2] 刘倩 LIANG Hai-xu;WANG Hai-bin;LIU Qian(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou,China 014040;Beijing Chaoyang District Center for Disease Control and Prevention;Chinese Preventive Medicine Association)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]北京市朝阳区疾病预防控制中心 [3]中华预防医学会

出　　处：《中国病原生物学杂志》2019年第12期1443-1447,1480,共6页Journal of Pathogen Biology

基　　金：北京市朝阳区科技计划项目(No.CYSF1721,CYSF172102)。

摘　　要：目的探讨北京市朝阳区汽感样病例(ILI)中的肠道病毒(EV)病原谱构成及分子流行病学特征,为流感防控提供理论依据。方法采集2016年1月-2018年12月,北京中日友好医院、首都医科大学附属北京朝阳医院2家哨点医院就诊的6327例ILI咽拭子标本,采用RT-PCT检测常见的11种呼吸道病原,对EV阳性标本采用普通PCR扩增病毒5-UTR片段,测序并进行基因分型。结果6327例ILI中,共检出EV阳性标本75例(1.19%),各年不同月份间及3年合计不同年龄组间EV阳性率比较差异有统计学意义(P均<0.05)。75例EV阳性标本其中28例分型成功,包括10例CV-A6.7例EV-68、6例CV-A10、2例CV-B1及CV-A2、CV-A4和CV-A5各1例。7例EV-68阳性标本进行聚类分析,5例A型,2例B型。结论2016-2018年北京市朝阳区ILI中EV病原谱主要包括CV-A6、EV-68、CV-A10、CV-B1、CV-A2、CV-A4和CV-A5,其中CV-A6、EV-68和CV-A10为优势病毒型,其中EV-68存在2个型别的共循环。Objective To investigate the pathogen sectrum and molecular epidemiological characteristcs of enterovir-us(EV)in cases of influenza-like iIness(ILI)in Chaoyang District,Beijing.Methods A total of 6,327 throat swabs from patients with ILI were collected at 2 sentinel hospitals(the Chaoyang Hospital of Capital Medical University and the China-Japan Friendship Hospital of the Ministry of Health)in Beijing from January 2016 to December 2018.Fluorescence quantitative RT-PCT was used to detect 11 respiratory viruses.EV-positive specimens were identified,and the 5-UTR fragment of EV was amplified using PCR,sequenced,and genotyped.Data were presented in tables and figures and the Chi-square test or Fisher's exact test used to compare between-group differences in percentages.Associations were consid-ered significant if the p-value was less than 5%.The data were analyzed using the software SPSS version 20.0.Results Of the 6,327 outpatients who sought treatment from 2016 to 2018,1,773 outpatients tested positive for a pathogen.Results indicated that 11 respiratory pathogens circulated in Bejing,and the most frequently detected pathogens were hu-man influenza virus(FluA/B)(16.15%;1,022/6,327),human rhinovirus(hRV)(3.38%;214/6,327),and mycoplas-ma(MP)(2.01%;127/6,327).Positivity for EV differed significantly by season and by age group during the 3 years studied(P<0.05).A total of 75(1.19%)EV-positive specimens were detected,28 of which were successfully classi-fied.CV-A6 was detected in 10 patients,EV-68 was detected in 7,CV-A10 was detected in 6,CV-B1 was detected in 2,CV-A2 was detected in 1,CV-A4 was detected in 1,and CV-A5 was detected in 1.A cluster analysis of 7 EV-68 positive samples indicated that type A was present in 5 and type B was present in 2.Conclusion The pathogen spectrum of EV in ILI in the Chaoyang District of Beijing from 2016-2018 included CV-A6,EV-68,CV-A10,CV-B1,CV-A2,CV-A4,and CV-A5.The dominant viruses were CV-A6,EV-68,and CV-A10.Two types of EV-68 were co-circulating in this region.

关 键 词：流感样病例 肠道病毒 感染谱 分子流行病学 

分 类 号：R373.2[医药卫生—病原生物学]