黑木耳无菌纳米粉对急性肝损伤小鼠的改善作用  

Effect of sterile black fungus nanoparticles on acute liver injury in mice

作　　者：徐博 吴畏难[2] 田晶 马宁 XU Bo;WU Wei-nan;TIAN Jing(School of Basic Medicine,Jilin Medical University,Jilin,Jilin Province 132013,China)

机构地区：[1]吉林医药学院基础医学院,吉林132013 [2]吉林市中心医院

出　　处：《中国公共卫生》2020年第1期85-88,共4页Chinese Journal of Public Health

基　　金：吉林省教育厅“十三五”科学技术项目（JJKH20180830KJ）;吉林省卫生计生科技创新项目（2017ZC035）;吉林市科技创新发展计划项目（20163048）

摘　　要：目的探讨黑木耳无菌纳米粉(SBFN)对乙醇所导致小鼠急性肝损伤的影响。方法70只昆明种小鼠随机分为正常对照组、乙醇模型组、阳性药组和SBFN低、中、高剂量组以及黑木耳普通粉组(SBFP)。采用一次性灌胃乙醇的方法造模。检测各组小鼠血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)和过氧化氢酶(CAT)活性;肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)水平;HE染色并观察肝组织病理改变;酶联免疫吸附法(ELISA)检测血清中肿瘤坏死因子(TNF-α)和白介素–6(IL-6)的表达;免疫组织化学法染色检测白介素–1β(IL-1β)的表达分布情况。结果与模型组相比,黑木耳无菌纳米粉和普通粉均可明显降低血清ALT、AST活性,提高CAT活力,下调TNF-α和IL-6的表达;降低肝组织中MDA含量,提高SOD、CAT活性;病理学光镜可见,肝脏组织形态结构改变明显减轻;免疫组化显示,肝脏IL-1β的表达被明显抑制。同等剂量下,黑木耳无菌纳米粉组以上各项要明显优于普通粉组。结论经过粒径改造的黑木耳无菌纳米粉对乙醇所致小鼠急性酒精性肝损伤具有明显的改善作用。Objective To study the effect of sterile black fungus nanoparticles(SBFN)on alcohol-induced liver injury in mice.Methods Totolly 70 Kunming mice were randomly divided into a normal control group,alcohol-induced injury model group,positive medication group,sterile black fungus powder(SBFP)group and low/moderate/high-dose SBFN group.After different treatments of once a day continuously for 7 days,all the mice were gavaged with 50%ethanol at the dose of 15 mL/kg to establish acute livery injury model,except for those of normal control mice with distilled water.For all the mice of different groups,contents of glutamic oxalacetic transaminase(GOT)and glutamic pyruvic transaminase(GPT)in plasma,superoxide dismutase(SOD),malondialdehyde(MDA)and catalase(CAT)in liver hemogenate were detected and compared;expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in plasma were measured with enzyme-linked immunosorbent assay(ELISA);the expression of interleukin-1 beta(IL-1β)in liver tissues was determined with immunohistochemical method.Pathogenic alterations in liver tissues were observed with haematoxylin-eosin(HE)staining.Results Compared with those of the mice of acute liver injury model group,reduced contents of GOT/GPT and expressions of TNF-α/IL-6 but increased CAT activity in plasma,decreased MDA but increased SOD and CAT in liver hemogenates,and declined expression of IL-1βin liver tissues were detected in the mice of SBFN and SBFP groups.Significantly alleviated morphological and structural changes in liver tissues were also observed in the mice of SBFN and SBFP groups in comparison with those in the mice of the injury model group.All the above liver injury-related indicators of the mice of SBFN groups were obviously better than those of the mice of SBFP groups under the treatment with the same dosage.Conclusion Sterile black fungus nanoparticles could obviously alleviate acute liver injury induced by ethanol in mice.

关 键 词：黑木耳无菌纳米粉 急性肝损伤 乙醇 

分 类 号：R994.4[医药卫生—毒理学]