神经元型一氧化氮合酶在先天性肥厚性幽门狭窄犬幽门的表达  

The expression of neuronal nitric oxide synthasein pylorus of congenital hypertrophic pyloric stenosis dog

作　　者：魏颖[1] 张又祥[1] WEI Ying;ZHANG Youxiang(Neonatalogy Department of Guangzhou First People s Hospital,Guangzhou 518180,China)

机构地区：[1]广州市第一人民医院新生儿科

出　　处：《广州医药》2020年第1期6-9,共4页Guangzhou Medical Journal

基　　金：广东省科技厅科技计划项目(2017ZC0317)

摘　　要：目的探讨先天性肥厚性幽门狭窄(congenital hypertrophic pyloric stenosis,IHPS)中神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)的表达情况,以进一步研究IHPS的发病机制。方法选取4只Beagle孕犬按3∶1分为模型组和对照组,自孕期第1天至分娩前1天,模型组孕犬每天腹腔注射一次L-NAME 6mg/(kg. d),对照组给予同样方式注射等量的生理盐水。所有新生犬均在生后第31天、38天、45天及52天分别称重,生后52天,选取模型组中体质量增长缓慢的新生犬和对照组新生犬各5只作为实验对象,取幽门组织HE染色后显微镜下测量幽门环肌厚度,免疫组化后运用图像分析技术测定nNOS表达量。结果与对照组新生犬比较,模型组中新生犬体质量增长慢,幽门环肌增厚,nNOS表达量减少,两组间有差异(P <0. 01,P <0. 01,P <0. 05)。结论幽门区nNOS表达减少与CHPS相关。Objective To further study the pathogenesis of infantile hypertrophic pyloric stenosis(IHPS)by analyzing the expression of neuronal nitric oxide synthase(nNOS)in pylorus of CHPS.Methods According to 3:1,four Beagle pregnant dogs were selected and divided into model group and control group,from the first day of pregnancy to the first day before delivery,L-NAME was intraperitoneally injected into pregnant dogs in the model group,dose for 6mg/(kg.d),while normal saline was intraperitoneally injected into pregnant dogs in the control group.The two groups newborn dogs were weighed at 31,38,45 and 52 days after birth,At 52 days after birth,5 newborn dogs were selected respectively between model group and control group as experimental subjects,obtained pyloric tissue for HE staining and measured the muscular thickness of pyloric,The expression of nNOS was determined by image analysis after immunohistochemistry.Results Compared with newborn dogs in the control group,newborn dogs in the model group had slow weight growth,increased pyloric annulus muscle thickness,and decreased expression of nNOS(P<0.01,P<0.01,P<0.05).Conclusion The decreased expression of nNOS in the pyloric region associated with IHPS.

关 键 词：神经元型一氧化氮合酶 婴儿肥厚性幽门狭窄 L-NAME BEAGLE犬 

分 类 号：R725.7[医药卫生—儿科] R-332[医药卫生—临床医学]