脱颗粒细胞与授精时间间隔对单精子卵胞浆内注射妊娠结局的影响  

Effect of degranulation cells and insemination time on pregnancy outcome of single sperm intracytoplasmic injection

作　　者：杨志伟 陶林林 李国臻 耿亚松 郑波 YANG Zhi-wei;TAO Lin-lin;LI Guo-zhen;GENG Ya-song;ZHENG Bo(Center of Reproductive Medicine,Xingtai Infertility Specialist Hospital/Xingtai Reproduction and Genetics Specialist Hospital,Xingtai Hebei 054000,P.R.China)

机构地区：[1]邢台不孕不育专科医院/邢台生殖与遗传专科医院生殖实验室

出　　处：《中国计划生育和妇产科》2020年第1期48-51,共4页Chinese Journal of Family Planning & Gynecotokology

摘　　要：目的探讨不同的脱颗粒细胞与卵胞浆内单精子显微注射技术(intracytoplasmic sperm injection,ICSI)时间间隔对新鲜周期ICSI妊娠结局的影响。方法回顾性分析邢台不孕不育专科医院/邢台生殖与遗传专科医院生殖中心2017~2018年行新鲜周期ICSI-ET患者的临床资料,共371个周期。根据脱颗粒细胞与ICSI时间间隔分为3组:A组(64个周期):t≤1 h组,B组(133个周期):1 h<t≤2 h组。C组(174个周期):t>2 h组。分别比较不同的脱颗粒细胞与ICSI时间间隔对妊娠结局的影响。结果3组基本资料、2 PN受精率、2 PN卵裂率、可用胚胎率比较,差异均无统计学意义(P>0.05)。A组和B组的妊娠率比较,差异无统计学意义(P>0.05),但两组和C组比较,差异均有统计学意义(P<0.05);A组的种植率高于B组和C组,差异均有统计学意义(P<0.05),但B组和C组比较,差异无统计学意义(P>0.05);3组流产率比较,差异无统计学意义(P>0.05)。结论卵母细胞脱颗粒后2 h内行ICSI能够获得较好的妊娠结局,且1 h内操作妊娠结局更好。Objective To investigate the effect of different intra-cytoplasmic sperm injection(ICSI)time intervals on the outcome of fresh-cycle ICSI pregnancy.Methods The clinical data of ICSI-ET patients in fresh cycles from 2017 to 2018 in Center of Reproductive Medicine,Xingtai Infertility Specialist Hospital/Xingtai Reproduction and Genetics Specialist Hospital were retrospectively analyzed.According to the time interval of degranulation cells and ICSI,it was divided into 3 groups:group A(64 cycles):t≤1 h group,group B(133 cycles):1 h<t≤2 h group.Group C(174 cycles):t>2 h group.The effects of different degranulation cells and ICSI intervals on pregnancy outcomes were compared.Results There was no significant difference in the basic data of the three groups(P>0.05).There was no significant difference in the 2 PN fertilization rate,2 PN cleavage rate,and available embryo rate in the 3 groups(P>0.05).There was no significant difference in the pregnancy rates between group A and group B,but there were statistically significant differences between the two groups and group C(P<0.05).The difference was statistically significant(P<0.05),but the difference between group B and group C was not statistically significant(P>0.05);the comparison of abortion rates among the three groups was not statistically significant(P>0.05).Conclusion ICSI can achieve better pregnancy outcomes within 2 h after oocyte degranulation,and it is better to manipulate pregnancy outcomes within 1 h.

关 键 词：脱颗粒细胞 卵胞浆内单精子注射 临床妊娠率 种植率 

分 类 号：R711.6[医药卫生—妇产科学] R321-33[医药卫生—临床医学]