丁基苯酞对老化的小胶质细胞诱导多巴胺能神经元损伤的影响  

Effects of d1-3-n-butylphthalide on the damage of dopaminergic neurons induced by age-associated activated microglia

作　　者：曹丹[1,2] 李晓红[1] 任艳[1] 罗晓光[1] 魏玲[2] CAO Dan;LI Xiaohong;REN Yan;LUO Xiaoguang;WEI Ling(Department of Neurology,The First Affiliated Hospital of China Medical University,Shenyang 110001,China;Department of Geriatrics,The Fourth People’s Hospital of Shenyang,Shenyang 11003,China)

机构地区：[1]中国医科大学第一临床医院神经内科,辽宁沈阳110001 [2]沈阳市第四人民医院老年病科,辽宁沈阳110031

出　　处：《西部医学》2020年第2期220-224,共5页Medical Journal of West China

摘　　要：目的探讨dl-3-丁基苯酞(NBP)衰老的小胶质细胞诱导的帕金森病细胞模型中的保护作用及机制。方法采用大鼠小胶质细胞原代培养和PC12细胞株的传代培养法,佛波酯诱导小胶质细胞异常活化,不同剂量的NBP预处理PC12细胞,然后加入低剂量鱼藤酮以建立与衰老小胶质细胞共育系统,模拟帕金森病体外细胞模型,在倒置显微镜下观察PC12细胞形态,同时用流式细胞仪检测各组PC12线粒体膜电位及细胞内活性氧监测,Annexin V-PI双染法监测PC12凋亡。结果与正常组相比,老化小胶质细胞共育的PC12细胞线粒体膜电位下降,细胞内ROS生成增多(P<0.01),而0.01~100μM的NBP均可不同程度地抑制这一变化。结论NBP通过抑制线粒体通透性、减少氧化应激等机制来降低PC12细胞凋亡,对神经元起保护作用。Objective To investigated the neuroprotective effectes of d1-3-n-Butylphthalide on the damage of dopaminergic neurons induced by age-associated activated microglia.Methods The primary culture of rat microglia and the subculture of PC12 cell line were used.Phorbol ester induced the abnormal activation of microglia.Different doses of NBP pretreated PC12 cells,and then low doses of rotenone were added to establish a co culture system with aging microglia,simulating the extracellular cell model of Parkinson’s disease.The morphology of PC12 cells was observed under the inverted microscope,and flow cytometry was used.The mitochondrial membrane potential and intracellular reactive oxygen species of PC12 were detected,and the apoptosis of PC12 was detected by annexin V PI double staining.Results Compared with the normal group,the mitochondrial membrane potential of PC12 cells co cultured with aged microglia decreased and ROS production increased(P<0.01),while NBP of 0.01-100μm could inhibit this change in varying degrees.Conclusion NBP can reduce apoptosis of PC12 cells and protect neurons by inhibiting mitochondrial permeability and reducing oxidative stress.

关 键 词：丁基苯酞 小胶质细胞 多巴胺能神经元 帕金森病 

分 类 号：R714.02[医药卫生—妇产科学]