α-萘乙酸对低温胁迫下油菜幼苗抗寒性的影响  

Regulation of α-naphthaleneacetic acid on cold resistance of Brassica campestris seedlings under low temperature stress

作　　者：罗丹瑜 张小花 李巧丽 王娟[1] 郑晟[1] 张腾国[1] LUO Dan-yu;ZHANG Xiao-hua;LI Qiao-li;WANG Juan;ZHENG Sheng;ZHANG Teng-guo(College of Life Sciences,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院

出　　处：《生态学杂志》2020年第1期99-109,共11页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31460099)资助

摘　　要：为探究α-萘乙酸(NAA)对植物抗寒性的影响,以白菜型冬油菜‘陇油6号’为试验材料,经4℃、NAA+4℃、NAA+4℃+DPI(NADPH氧化酶抑制剂)、NAA+4℃+DMTU(H2O2清除剂)、NAA+4℃+U0126(MAPK抑制剂)和NAA+4℃+Tungstate(NO生成抑制剂)处理后,研究其对‘陇油6号’油菜的活性氧(H2O2和O2-·)含量,抗氧化酶活性,丙二醛(MDA)、可溶性糖、脯氨酸和叶绿素含量,抗氧化酶基因(APX、CAT、GR、SOD)、Rboh A-F、MAPK3/4/6、CBF和ICE1基因表达量的影响。结果表明:与4℃低温处理相比,NAA+4℃处理下油菜根系中的细胞活性、H2O2和O2-·含量以及叶片中的MDA含量均降低;根系中的抗氧化酶(CAT、SOD、APX和POD)活性、叶片中的可溶性糖及脯氨酸含量、叶绿素含量、上述相关基因的表达量均升高,说明α-萘乙酸处理油菜可显著提高低温胁迫下油菜幼苗的抗氧化能力、光合能力和相关基因的表达,增强油菜幼苗的抗寒性。与NAA+4℃处理相比,NAA+4℃+抑制剂(DPI、DMTU、U0126和Tungstate)处理下油菜幼苗中叶绿素含量、抗氧化酶基因表达量、Rboh A-F、MAPK3/4/6、CBF和ICE1基因表达量均呈不同程度降低,说明H2O2和NO信号分子、NADPH氧化酶和MAP激酶级联途径均参与了α-萘乙酸增强油菜幼苗耐寒性过程的调控。We investigated the regulation of α-naphthaleneacetic acid(NAA) on cold resistance of Brassica campestris variety ‘Longyou 6’seedlings by measuring parameters,including reactive oxygen(H2O2 and O2·-) content,antioxidant enzyme activities,contents of malondialdehyde(MDA),soluble sugar,proline and chlorophyll,the expression level of antioxidant enzymes genes(APX,CAT,GR,SOD),RbohA-F,MAPK3/4/6,CBF and ICE1 genes. Six treatments were applied to both leave and root tissues: 4 ℃,NAA+4 ℃,NAA+4 ℃ +DPI(NADPH oxidase inhibitor),NAA+4 ℃ +DMTU(H2O2 scavenger),NAA+4 ℃ +U0126(MAPK inhibitor),and NAA+ 4 ℃ + Tungstate(NO production inhibitor). The results showed that cell viability,H2O2 and O2·-contents,and MDA content in leaves decreased under NAA+4 ℃ treatment compared with 4 ℃ treatment. Antioxidant enzymes(CAT,SOD,APX and POD) in roots as well as soluble sugar,proline,chlorophyll contents and related genes expression in leaves increased.These results indicated that Brassica campestris seedlings treated with α-naphthaleneacetic acid could significantly improve their antioxidant capacity,photosynthetic capacity,and the expression of related genes,and consequently enhance their cold resistance. Compared with NAA + 4 ℃treatment,chlorophyll content,antioxidant enzymes genes(APX,CAT,GR,SOD),RbohA-F,MAPK3/4/6,CBF and ICE1 decreased in seedlings treated with NAA + 4 ℃ +inhibitors(DPI,DMTU,U0126 and Tungstate) to different extents,indicating that H2O2 and NO signaling molecules,NADPH oxidase and MAP kinase cascades are all involved in the regulation of α-naphthaleneacetic acid on cold resistance of Brassica campestris seedlings.

关 键 词：白菜型油菜 Α-萘乙酸 低温胁迫 抗氧化酶 基因表达量 

分 类 号：S565.4[农业科学—作物学]