利用转录组测序分析外源GA3解除大蒜气生鳞茎休眠相关基因  

Analysis of Differentially Expressed Genes of Exogenous GA3 Releasing Aerial Bulbs Dormancy in Garlic Based on Transcriptome Sequencing

作　　者：董玉惠[1] 王立霞 顾启玉 刘中良[2] 孙秀东[1] 刘世琦[1] DONG Yuhui;WANG Lixia;GU Qiyu;LIU Zhongliang;SUN Xiudong;LIU Shiqi(College of Horticulture Science and Engineering,Shandong Agricultural University,Shandong garlic Engineering Research Center,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China;Tai’an Academy of Agricultural Sciences,Tai’an,Shandong 271000,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东省大蒜工程技术研究中心,作物生物学国家重点试验室,山东泰安271018 [2]泰安市农业科学研究院,山东泰安271000

出　　处：《园艺学报》2019年第12期2335-2346,共12页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目（31772323）;山东省自然科学基金项目（2R2016CM33)

摘　　要：利用外源GA3打破’金乡’大蒜气生鳞茎休眠,采用高通量测序技术对休眠气生鳞茎和GA3处理气生鳞茎进行转录组测序,分析筛选响应外源GA3的差异表达基因,探究差异表达基因与GA3解除气生鳞茎休眠的关系。结果表明:外源GA3可有效打破气生鳞茎休眠;通过转录组测序得到上调差异基因4588个,下调差异基因55857个;28107个差异表达基因在KEGG中获得注释,其中在代谢途径中获得注释的差异基因最多,其次为遗传信息处理途径。差异表达基因较多的富集于植物激素信号转导途径且富集结果显著;筛选出参与GA、ABA、ZR信号转导的显著差异表达基因GAI、SLR1、PP2C等,其表达情况与气生鳞茎内源激素含量的变化情况一致,推测外源GA3可通过抑制GA信号途径负调控因子DELLA基因的表达,激活赤霉素的生物合成及信号转导过程,使气生鳞茎休眠解除,同时PYL的表达量显著降低,从而使得脱落酸含量相对降低,有利于休眠解除;qRT-PCR验证结果显示测序结果和实际结果一致。Exogenous GA3 was used to break the dormancy of Jinxiang aerial bulbs.High throughput sequencing technology was used to sequence the transcriptome of dormant and GA3-treated aerial bulbs.To analyze and screen differentially expressed genes in response to exogenous GA3,and to explore the relationship between differentially expressed genes and the release of aerial bulb dormancy by GA3.The results showed that exogenous GA3 could effectively break the dormancy of aerial bulbs;4588 up-regulated genes and 55857 down-regulated genes were obtained by transcriptome sequencing;28107 differentially expressed genes were annotated in KEGG;among which the most differentially expressed genes were annotated in metabolic pathway;followed by genetic information processing pathway.More differentially expressed genes were enriched in plant hormone signal transduction pathway and the enrichment results were significant.Significant differentially expressed genes GAI,SLR1,PP2 C involved in GA,ABA and ZR signal transduction were screened out.Their expressiones were consistent with the change of endogenous hormone content in aerial bulbs.It was speculated that exogenous GA3 could activate the biosynthesis and signal transduction of gibberellin by inhibiting the expression of DELLA gene,a negative regulator of GA signal pathway,so as to release dormancy of aerial bulbs.At the same time,the significant decrease of PYL expression resulted in a relative decrease of abscisic acid content,which was conducive to the relief of dormancy.The results of qRT-PCR showed that the sequencing results were consistent with the actual results,which indicated that the data of transcriptome sequencing were true and reliable.

关 键 词：大蒜 转录组测序 外源GA3 气生鳞茎 休眠解除 差异表达基因 

分 类 号：S633.4[农业科学—蔬菜学]