根肿菌Williams系统中各单孢生理小种的获得与鉴定  

Obtaining and Identification of Single-spore Physiological Races of Plasmodiophora brassicae in Williams System

作　　者：张晶 武月 冯辉[1] 葛文杰 刘旭垚 吕明灿 王艺琏 冀瑞琴[1] ZHANG Jing;WU Yue;FENG Hui;GE Wenjie;LIU Xuyao;LÜMingcan;WANG Yilian;JI Ruiqin(Liaoning Provincial Key Laboratory of Genetics and Breeding of Cruciferous Vegetable Crops,Department of Horticulture,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学园艺学院,辽宁省十字花科蔬菜遗传育种重点室验室

出　　处：《园艺学报》2019年第12期2415-2422,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目（31972412）;辽宁省自然科学基金项目（2019-MS-283）

摘　　要：将根肿菌单孢分离技术和Williams生理小种鉴定方法结合,对中国9个大白菜主要产区的根肿菌进行单孢分离及生理小种鉴定。共获得281个单孢菌株,通过Williams鉴定,这些单孢菌株分别属于该系统中除5号生理小种之外的15个生理小种类型。其中4号生理小种所占比例最大,共191个,占67.97%,9个地区的优势生理小种均为4号小种。We combined the single spore isolation technology and Williams identification method to isolate single spores of Plasmodiophora brassicae from nine major Chinese cabbage producing areas in China and identify their physiological races,to obtain more types of single-spore physiological races.A total of 281 single-spore stains were obtained and identified by Williams identification,these single spores belong to 15 types of physiological races other than No.5 physiological race in the system.Among them,191 single-spores were of the No.4 physiological race accounting for 67.97%.The dominant physiological race in the 9 regions was No.4.

关 键 词：十字花科蔬菜作物 根肿病 单孢分离技术 生理小种鉴定 

分 类 号：S634[农业科学—蔬菜学]