山西省某焦化厂下风向居民尿中多环芳烃羟基代谢产物与血常规指标的关联  

Association between polycyclic aromatic hydrocarbon hydroxy metabolites in urine and blood routine indices of downwind residents of a coking enterprise

作　　者：张超 周小林[1] 张婷 孟倩倩[1] 薛振伟[1] 刘占旗[1] 安全[1] 李炜宾[1] ZHANG Chao;ZHOU Xiao-lin;ZHANG Ting;MENG Qian-qian;XUE Zhen-wei;LIU Zhan-qi;AN Quan;LI Wei-bin(State Environmental Protection Key Laboratory of Environment and Health,China Institute for Radiation Protection,Taiyuan,Shanxi 030000,China)

机构地区：[1]中国辐射防护研究院国家环境保护环境与健康重点实验室

出　　处：《环境与职业医学》2019年第12期1125-1130,共6页Journal of Environmental and Occupational Medicine

摘　　要：[背景]多环芳烃(PAHs)是持久性有机污染物,主要来源于烧烤、烟草、烟熏食物以及焦煤、石油等有机化合物的热解和不完全燃烧,可诱导机体发生恶性病变。目前对PAHs的探讨主要集中于职业人群,对于污染企业周边居民是否存在暴露风险的研究较少。[目的]探讨非职业人群PAHs内暴露水平及其与血常规指标的关联。[方法]于2018年7月对山西省某焦化企业下风向居住的255名村民进行问卷调查,并收集血样和尿样各255份。使用全自动血细胞分析仪对血常规指标进行检测,采用高效液相色谱检测方法对尿样中8种PAHs羟基代谢产物(1-羟基萘、2-羟基萘、2-羟基芴、9-羟基芴、1-羟基菲、2-羟基菲、1-羟基芘、3-羟基苯并[a]芘)进行分析。把研究对象血常规指标作为应变量,调整性别、年龄、体重指数(BMI)、吸烟、饮酒等影响因素,以8种PAHs羟基代谢产物为自变量,进行多元线性逐步回归分析。[结果]共调查255人,其中男性62人,女性193人。异常率较高的血常规指标为平均血红蛋白浓度(24.31%)、淋巴细胞比率(19.22%)、单核细胞比率(16.86%)、血红蛋白浓度(13.33%)、平均血红蛋白含量(12.55%)、中性粒细胞比率(12.16%)。尿中PAHs羟基代谢产物1-羟基萘、2-羟基萘、2-羟基芴、9-羟基芴、1-羟基菲、2-羟基菲、1-羟基芘、3-羟基苯并[a]芘检出率均高于85%,检出水平中位数分别为0.98、0.57、0.33、8.13、0.11、0.13、0.14、0.18μmol/mol(以肌酐计)。调整相关混杂因素后,多元线性回归模型显示被调查人群尿中1-羟基萘、1-羟基芘的检出水平与机体血红蛋白含量呈负相关(P<0.05);2-羟基萘、1-羟基芘的检出水平与机体淋巴细胞比率呈负相关(P<0.05)。[结论]非职业人群低剂量暴露于PAHs可能导致机体发生血象的早期改变。[Background]Polycyclic aromatic hydrocarbons(PAHs)are persistent organic pollutants which originate from barbecue,tobacco,smoked food,and pyrolysis and incomplete combustion of organic compounds such as coking coal and petroleum,and can induce malignant lesions in the body.At present,discussions on PAHs mainly focus on occupational groups,and there are few studies on whether there are exposure risks for residents around polluting enterprises.[Objective]The study aims to investigate the association between PAHs internal exposure levels and blood routine indices in a non-occupational group.[Methods]In July 2018,an epidemiological survey was conducted among 255 villagers living downwind of a coking enterprise in Shanxi Province,and their blood samples(n=255)and urine samples(n=255)were collected.The blood routine indicators were detected using an automatic blood cell analyzer.The PAHs hydroxy metabolites(1-hydroxynaphthalene,2-hydroxynaphthalene,2-hydroxyfluorene,9-hydroxyfluorene,1-hydroxyphenanthrene,2-hydroxyphenanthrene,1-hydroxyperene,and 3-hydroxybenzo[a]pyrene)were analyzed by high performance liquid chromatography.Multiple stepwise linear regression analysis was conducted with blood routine indices as dependent variablesand eight PAHs hydroxy metabolites as independent variables after adjusting for sex,age,body mass index,smoking,and drinking.[Results]A total of 255 residents were surveyed,including 62 males and 193 females.The abnormal rates of mean hemoglobin concentration(24.31%),lymphocyte ratio(19.22%),monocyte ratio(16.86%),hemoglobin contentration(13.33%),mean hemoglobin content(12.55%),and neutrophil ratio(12.16%)were relatively high.The detection rates of PAHs hydroxy metabolites 1-hydroxynaphthalene,2-hydroxynaphthalene,2-hydroxyfluorene,9-hydroxyfluorene,1-hydroxyphenanthrene,2-hydroxyphenanthrene,1-hydroxyperene,and 3-hydroxybenzo[a]pyrene were all above 85%,and the median concentrations were 0.98,0.57,0.33,8.13,0.11,0.13,0.14,and 0.18μmol/mol(in creatinine),repectively.After adjusting for selec

关 键 词：多环芳烃 血红蛋白含量 淋巴细胞比率 多元线性逐步回归分析 

分 类 号：R127[医药卫生—环境卫生学]