FADS2 rs3834458基因多态性与中国汉族人群冠心病易感性的相关性研究  

Association between FADS2 rs3834458 Gene Polymorphism and the Susceptibility to Coronary Artery Disease

作　　者：徐颖誉 赵真真 刘守胜 肖颖朴 苗梦媛 辛永宁[1,5] XU Ying-yu;ZHAO Zhen-zhen;LIU Shou-shen;XIAO Ying-pu;M IAO Meng-yuan;XIN Yong-ning(The Affiliated Qingdao Municipal Hospital of Qingdao University,Qingdao,Shandong,266011,China;Central Laboratories,Qingdao Municipal Hospital,Qingdao,Shandong,266071,China;Dalian Medical University,Dalian,Liaoning,116044,China;Nanjing Medical University,Nanjing,Jiangsu,211166,China;Department of In fectious Disease,Qingdao Municipal Hospital,Qingdao,Shandong,266011,China)

机构地区：[1]青岛大学附属青岛市市立医院,山东青岛266011 [2]青岛市市立医院中心实验室,山东青岛266071 [3]大连医科大学,辽宁大连116044 [4]南京医科大学,江苏南京211166 [5]青岛市市立医院感染性疾病科,山东青岛266011

出　　处：《现代生物医学进展》2019年第22期4348-4352,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31770837)

摘　　要：目的:探讨脂肪酸去饱和酶2(FADS2)基因rs3834458位点多态性与中国汉族人群冠心病易感性的关系。方法:随机抽取青岛地区汉族人群中149名无血缘关系的健康体检人群为对照组以及我院收治的192例冠心病患者为冠心病组。采用聚合酶链反应(PCR)对健康人群、冠心病患者进行FADS2 rs3834458基因分型,检测受试者的生化指标,采用x2检验、t检验、Wilcoxon秩和检验和Logistic回归进行统计学分析。结果:冠心病组男性比例、年龄、体重指数(BMI)、血糖(GLU)、总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白(LDL-C)、丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST)、γ-谷氨酰转移酶(GGT)、碱性磷酸酶(ALP)均显著高于对照组(P<0.05),而高密度脂蛋白(HDL-C)水平明显低于对照组(P<0.05)。两组间的基因型分布和等位基因频率比较无显著差异(P>0.05)。性别(男性)、年龄、GLU、TC和HDL-C(<1.00 mmol/L)是冠心病发生的独立危险因素(OR=3.57,1.14,1.34,3.50,2.89)。FADS2rs3834458有T/T、T/del、del/del三种基因型,而T等位基因不是冠心病发生的独立危险因素(P=0.641)。结论:FADS2 rs3834458基因多态性与中国汉族人群中冠心病发病风险无明显相关性。Objective: To investigate the relationship between the polymorphism of fatty acid desaturase 2(FADS2) rs3834458 and susceptibility to coronary artery disease. Methods: 149 unrelated bloody health examinations in the Han population of Qingdao were randomly selected as the control group and 192 patients with coronary artery disease admitted to our hospital were randomly selected as the coronary artery disease group. PCR was used to genotype FADS2 rs3834458 in both groups. At the same time, the clinical data of were collected, and the biochemical indexes were tested. The above data were analyzed by t test, Wilcoxon rank sum test, chi-square test and Logistic regression. Results: The male proportion, Age, Body mass index(BMI), blood glucose(GLU), total cholesterol(TC),triglycerides(TG), low-density lipoprotein(LDL-C), alanine aminotransferase(ALT), aspartate aminotransferase(AST),gammon-glutamyl transferase(GGT), alkaline phosphatase(ALP) of coronary artery disease group were all higher than those of healthy control group(P<0.05), while the level of high-density lipoprotein(HDL-C) was significantly lower than that of the control group(P<0.05). There was no significant difference in the genotype distribution and allele frequency between the two groups(P>0.05). Gender(male), age, GLU, TC and HDL-C(<1.00 mmol/L) were independent risk factors for coronary artery disease group(OR=3.57, 1.14, 1.34,3.50, 2.89). FADS2 rs3834458 has three genotypes: T/T, T/del, del/del, and the T allele is not an independent risk factor for coronary artery disease(P=0.641). Conclusion: There was no significant correlation between FADS2 rs3834458 polymorphism and the risk of coronary artery disease in Chinese Han population.

关 键 词：脂肪酸去饱和酶2 冠心病 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]