基于UPLC-TQ-MS对苦豆子中生物碱类成分定性分析方法的研究  

Qualitative analysis of chemical constituents of alkaloids in Sophora alopecuroides L.based on UPLC-TQ-MS

作　　者：陈龙 曲丽媛[2] 刘雪滢 王航宇 张珂 王金辉 CHEN Long;QU Liyuan;LIU Xueying;WANG Hangyu;ZHANG Ke;WANG Jinhui(School of Pharmacy,Shihezi University/Key Laboratory of Xinjiang Plant Resources and Utilization,Ministry of Education,Shihezi,Xinjiang 832002,China;School of Pharmacy,Harbin Medical University,Harbin,Heilongjiang 150081,China;Shenzhen Honghui Biopharmaceutical CO.,Ltd,Shenzhen,Guangdong 518000,China)

机构地区：[1]石河子大学药学院/新疆植物药资源利用教育部重点实验室,新疆石河子832002 [2]哈尔滨医科大学药学院,黑龙江哈尔滨150081 [3]深圳弘汇医药科技有限公司,广东深圳518000

出　　处：《石河子大学学报（自然科学版）》2020年第1期121-127,共7页Journal of Shihezi University(Natural Science)

基　　金：国家重大专项新药创制项目(2018ZX09735005);石河子大学高层次人才科研启动项目(RCSX201715)

摘　　要：目的建立一种UPLC-TQ-MS对苦豆子中生物碱类成分定性分析的方法,可以对苦豆子药材中生物碱类成分进行快速鉴定。方法以UPLC-TQ-MS方法,结合相关文献和数据库筛查,依据其精确分子质量、特征离子、MS/MS裂解规律和色谱保留行为对苦豆子中生物碱类化学成分进行定性鉴定。结果共鉴定出苦豆子中4类生物碱并总结出其中3类质谱裂解规律,同时基于其裂解规律分析出7种未知化合物。结论建立的方法可以快速定性分析苦豆子中生物碱类成分,为研究苦豆子化学成分提供了可靠依据。Objective To establish a qualitative analysis method for alkaloids in Sophora alopecuroides by UPLC-TQ-MS,and to quickly identify the alkaloids in Sophora alopecuroides.Methods According to experimental results acquired by UPLC-TQ-MS and combined with relevant literature and database screening,based on the exact molecular mass,characteristic ions,MS/MS cleavage rules and chromatographic retention behavior of alkaloids in Sophora alopecuroides,the chemical constituents of alkaloids in beans were qualitatively identified.Results Four kinds of alkaloids in Sophora alopecuroides were identified and cleavaage rules of three of them were summarized by mass spectrometry.What's more,seven unknown compounds were analyzed based on their cleavage rules.Conclusion The established method can quickly and qualitatively analyze the alkaloids in Sophora alopecuroides,which provides a reliable basis for studying the chemical constituents of Sophora alopecuroides.

关 键 词：UPLC-TQ-MS 苦豆子 定性分析 生物碱成分 

分 类 号：R917[医药卫生—药物分析学]