干燥综合征合并原发性胆汁性肝硬化患者的外周血Th17/Treg细胞百分比及临床特征  

Percentage of Th17/Treg cells in peripheral blood of patients with Sjogren′s syndrome and primary biliary cirrhosis and their clinical characteristics

作　　者：郇稳史 丽璞 HUAN Wen;SHI Li-pu(Department of Rheumatology and Immunology,People′s Hospital of Zhengzhou,Henan 450003,China;Department of Rheumatology and Immunology,Henan Provincial People′s Hospital,Zhengzhou,Henan 450008,China)

机构地区：[1]郑州人民医院风湿免疫科,450003 [2]河南省人民医院风湿免疫科

出　　处：《肝脏》2020年第2期155-157,共3页Chinese Hepatology

基　　金：河南省科技厅项目(182102310546)。

摘　　要：目的通过对SS合并PBC患者中外周血Th17和Treg细胞的检测,分析其临床特征,并探明Th17、Treg细胞对SS合并PBC患者的影响。方法选择2012年3月至2018年2月期间郑州人民医院收治的SS合并PBC患者22例,SS患者48例,选取健康志愿者68名。比较3组研究对象的肝功能指标、Th17、Treg和TH17/Treg水平。组间比较采用t检验或Kruskal-Wallis H检验;计数资料以百分比表示,组间比较采用卡方检验。结果SS合并PBC组出现黄疸、皮肤瘙痒、腹水、消化道出血、口干、食管静脉曲张、发热及眼部干涩等主要临床表现为20例(90.9%,20/22),高于对照组37例(77.1%,37/48),(P<0.05)。而体质量减轻的患者SS组(46例,95.8%)明显多于SS合并PBC组(17例,77.3%),(P<0.05)。SS合并PBC组患者ALT、AST、碱性磷酸酶(ALP)、γ-谷氨酰胺酶(γ-GT)和TBil均高于SS组和健康组(P<0.05)。SS合并PBC组患者Th17细胞百分比为[16.6(10.1,22.5)%],明显高于SS组[9.4(7.0,13.0)%,P<0.05]及健康组[4.9(4.3,9.8)%,P<0.05];SS合并PBC组Th17/Treg细胞比值[0.52(0.42,0.62)]均明显高于SS组[0.25(0.14,0.37),P<0.05]及健康组[0.22(0.13,0.26),P<0.05]。结论SS合并PBC患者外周血Th17/Treg细胞水平及其比值明显失衡,提示Th17细胞在很大程度上参与了致病过程。此外,Th17/Treg比值与某些肝功能指标有一定的相关性。Objective To detect the levels of T helper 17(Th17)and regulatory T(Treg)cells in peripheral blood of Sjogren's syndrome(SS)patients combined with primary biliary cirrhosis(PBC),to analyze their clinical characteristics,and to investigate the effects of Th17/Treg cells on these patients.Methods A total of 22 patients with SS complicated with PBC aged(54.1±9.6)years,including 7 males and 15 females,and 48 SS patients aged(56.7±10.2)years,including 10 males and 38 females,in our hospital from March 2012 to February 2018 were enrolled.And 68 healthy volunteers were enrolled,including 16 males and 52 females,with an average age of(55.2±4.5)years.The measurement data of normal distribution are expressed by mean±standard deviation,the measurement data of non-normal distribution are expressed by median(percentage 25,percentage 75)],and the comparison between the groups is conducted by t test or Kruskal-Wallis H test.Counting data are expressed as percentages,and comparisons between the groups are made by chi-square test.Results There were 20 cases(90.9%)in the observation group(SS combined with PBC)with the main clinical manifestations of jaundice,skin pruritus,ascites,gastrointestinal hemorrhage,dry mouth,esophageal varices,fever and dry eyes,higher than 37 cases(77.1%)in the control group(SS).However,the number of patients with weight loss in the control group(46,95.8%)was significantly higher than that in the observation group(17,77.3%).The alanine aminotransferase,aspartate transaminase,alkaline phosphatase,γ-glutaminase and total bilirubin in the observation group were higher than those in the control group and the healthy group(P<0.05).The percentage of Th17 cells in the observation group was 16.6%(10.1%,22.5%),significantly higher than that in the control group[9.4%(7.0%,13.0%),P<0.05]and the healthy group[4.9%(4.3%,9.8%%,P<0.05].The ratio of Th17/Treg cells in observation group[0.52(0.42,0.62)]was significantly higher than that in control group[0.25(0.14,0.37),P<0.05]and healthy group[0.22(0.13,0.26),P<0.05

关 键 词：干燥综合征 原发性胆汁性肝硬化 调节性T淋巴细胞 辅助性T淋巴细胞17 

分 类 号：I20[文学—中国文学]