基于线粒体D-loop区序列和Cyt b基因的鲟鱼群体遗传多样性分析  

Genetic Diversity of Acipenser Population Revealed by Cyt b Gene and D-loop Sequence

作　　者：孔杰 刘伟 李世凯 周洲 赵凤 KONG Jie;LIU Wei;LI Shi-kai;ZHOU Zhou;ZHAO Feng(Fisheries Research Institute,Guizhou Academy of Aquaculture Sciences,Guizhou Guiyang 550025,China)

机构地区：[1]贵州省农业科学院水产研究所

出　　处：《西南农业学报》2020年第1期206-210,共5页Southwest China Journal of Agricultural Sciences

基　　金：现代农业产业技术体系专项(CARS-46);贵州省科技计划项目“贵州养殖鲟鱼种质鉴定方法的研究与应用”[黔科合支撑(2016)2584];贵州省科研机构服务企业行动计划项目“鲟鱼繁养技术升级与精细加工技术创新”[黔科合平台人才(2016)5714];贵州省科技支撑计划课题项目“鲟鱼精子超低温冷冻保存技术研究及应用”[黔科合NY(2015)3003-2]

摘　　要：【目的】为评估施氏鲜和西伯利亚歸后备亲鱼群体的遗传多样性,为歸鱼的遗传选育提供参考。【方法】以施氏鳏和西伯利亚歸亲鱼群体为研究对象,进行了线粒体控制区(D-loop)全序列和细胞色素6(Cyt 6)基因序列对比分析,探讨2标记对歸鱼遗传多样性分析结果的差异与关系。【结果】西伯利亚歸和施氏MCyti和D-loop的A+T含量均高于G+C含量,碱基组成具有偏倚性。Cyt 6基因在西伯利亚歸中定义单倍型3个,多态性位点5个,单倍型多样性0.151,核甘酸多样性0.00036;在施氏歸中定义单倍型4个,多态性位点4个,单倍型多样性0.612,核昔酸多样性0.00114o D-loop在西伯利亚歸中定义单倍型3个,多态性位点2个,单倍型多样性0.385,核昔酸多样性0.00040;在施氏歸中定义单倍型5个,多态性位点55个,单倍型多样性0.707,核昔酸多样性0.02242o【结论】西伯利亚歸和施氏歸亲鱼群体的遗传多样性匮乏,尤其在利用西伯利亚鳏亲鱼进行繁殖育苗时要注意近交的不利影响。[Objective]The study aimed to evaluate genetic diversity of Acipenser baerii and A.schrenkii populations,and offer a reference for genetic breeding.[Method]In this paper the authors took the reserved parent fish broodstocks of A.baerii and A.schrenkii populations as the research object,by comparison of imtochondrial DNA cytochrome b(Cyt 6)gene and control region(D-loop)sequences to investigate the relation and difference genetic diversity relation of A.Baerii and A.Schrenkii with the two molecular markers.[Result]Whatever A.baerii or A.schrenkii,the content of A+T base pairs were greater than that of the G+C base pairs in both Cyt b gene and D-loop sequences,indicating that the base composition was biased.Three haplotypes were defined and five polymorphic sites(S)were detected in Cyt b gene of 4.baerii,while its haplotype diversity(H&)was 0.151 and nucleotide diversity(tf)was 0.00036.Four haplotypes were defined and four polymorphic sites were detected in Cyt b gene sequences of A.fiaezii,while its haplotype diversity was 0.612 and nucleotide diversity was 0.00114.Three haplotypes were defined and two polymorphic sites were detected in D-loop sequences of A.baerii,while its haplotype diversity was 0.385 and nucleotide diversity was 0.00040;Five haplotypes were defined and 55 polymorphic sites were detected in D-loop sequences of A.baerii,while its haplotype diversity was 0.707 and nucleotide diversity was 0.02242.[Conclusion]The results indicated that a marked low genetic diversity in reserved parent fish broodstocks,and more attention should be paid to avoiding the inbred risk in sturgeon fry production.

关 键 词：西伯利亚歸 施氏M Cyt i D-LOOP 遗传多样性 

分 类 号：S917[农业科学—水产科学]