紫萁孢子叶组织培养技术研究  

Tissue culture of osmunda spore leaves

作　　者：孙晓丹 李春鹏 董然[1,2] 王克凤 周煊 SUN Xiaodan;LI Chunpeng;DONG Ran;WANG Kefeng;ZHOU xuan(College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118,China;Center of the Ecological Recourse Development of Changbai Mountain,Changchun Sci-Tech University,Changchun,Jilin 130600,China)

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]长春科技学院长白山生态资源开发工程研究中心,吉林长春130600

出　　处：《西北农林科技大学学报（自然科学版）》2020年第3期107-114,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：吉林省科技厅科技支撑计划项目“特殊蔬菜(含山野菜类)品种选育、栽培技术研究”(20180201079NY)。

摘　　要：【目的】建立紫萁(Osmunda japonica Thunb.)孢子叶组织培养快繁体系,筛选其适宜的组织培养条件。【方法】以紫萁孢子叶穗为外植体,研究乙醇和HgCl 2处理不同时间对外植体的消毒效果;在加入0.3 mg/L NAA的1/4MS培养基中,再分别加入0.5 mg/L的2,4-D、IBA和KT,比较各处理对原叶体的诱导效果;在1/2MS基础培养基上,采用L 9(34)正交试验设计,探讨不同质量浓度KT、GA 3、NAA和6-BA组合对原叶体增殖的影响;同时,研究光合酵素稀释300,500和700倍对紫萁孢子体诱导的影响,以及1/2MS培养基中加入0.1,0.5,1.0 mg/L IBA或NAA对紫萁组培苗生根的影响。【结果】使用1 g/L HgCl 2对紫萁孢子叶穗消毒8 min的效果较好,萌发率为61.11%;紫萁原叶体最适宜的诱导培养基为1/4MS+KT 0.5 mg/L+NAA 0.3 mg/L,诱导率为88.55%;紫萁原叶体最适宜的增殖培养基为1/2MS+KT 5 mg/L+GA 33 mg/L+NAA 1 mg/L,增殖系数为9.6;稀释500倍的光合酵素对紫萁孢子体苗的转化及生长有促进作用,孢子体转化率为57.23%,孢子体高度为2.33 cm;1/2MS培养基中加入0.5 mg/L IBA,紫萁组培苗生根率与长势俱佳,生根率可达93.33%。【结论】初步建立了紫萁孢子适宜的组织培养体系,在相应条件下组培苗长势良好。【Objective】This study aimed to establish a rapid propagation system for tissue culture of osmunda spore leaves and screen suitable conditions.【Method】The spores of osmunda were used as explants to study the disinfection effect of ethanol and HgCl 2 on explants with different treatment times.In the 1/4 MS culture medium with 0.3 mg/L NAA,2,4-d,IBA and KT at 0.5 mg/L were added,respectively,and the induction effects on protoplasts were compared.On 1/2MS basal medium,the L 9(34)orthogonal design was used to investigate the effects of KT,GA 3,NAA and 6-BA at different concentrations on proliferation of protoplasts.The effects of 300,500 and 700 times dilution of photosynthetic enzymes on induction of osmunda sporophyte and the effect of 0.1,0.5,1.0 mg/L IBA or NAA on rooting of osmunda tissue culture seedlings in 1/2MS medium were also investigated.【Result】Sterilization with 1 g/L HgCl 2 for 8 min was better with germination rate of 61.11%.The most suitable induction medium for osmunda protoplasts was 1/4MS+KT 0.5 mg/L+NAA 0.3 mg/L with induction rate of 88.55%.The most suitable proliferation medium for osmunda protoplasts was 1/2MS+KT 5 mg/L+GA 33 mg/L+NAA 1 mg/L with proliferation coefficient of 9.6.The photosynthetic enzyme diluted by 500 times promoted the transformation and growth of osmunda spore seedlings with sporophytic transformation rate of 57.23%and spore body height of 2.33 cm.Adding 0.5 mg/L IBA to 1/2MS medium improved rooting rate and growth of osmunda tissue culture seedlings and the rooting rate reached 93.33%.【Conclusion】A suitable tissue culture system for osmunda spores was initially established,and the tissue culture seedlings grew well under corresponding conditions.

关 键 词：紫萁 组织培养 孢子繁殖 激素诱导 

分 类 号：S647.043[农业科学—蔬菜学]