新型冠状病毒核酸临床检测要点及经验  

Clinical points and experience in nucleic acid testing of SARS-CoV-2

作　　者：钟慧钰 赵珍珍[1] 宋兴勃[1] 陆小军[1] 王旻晋[1] 周易[1] 宋佳佳 刘堂喻亨 巫丽娟[1] 周汶静[1] 叶远馨[1] 斯艳君 周燕虹[1] 陶昕彤 王念[1] 应斌武[1] ZHONG Huiyu;ZHAO Zhenzhen;SONG Xingbo;LU Xiaojun;WANG Minjin;ZHOU Yi;SONG Jiajia;LIUTANG Yuheng;WU Lijuan;ZHOU Wenjing;YE Yuanxin;SI Yanjun;ZHOU Yanhong;TAO Xintong;WANG Nian;YING Binwu(Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西医院实验医学科

出　　处：《国际检验医学杂志》2020年第5期523-526,共4页International Journal of Laboratory Medicine

基　　金：四川省科技厅应对新型冠状病毒科技攻关应急项目(2020YFS0004)

摘　　要：2019年12月以来,新型冠状病毒感染引起的肺炎病例数快速攀升。因患者体内病毒核酸的存在是目前唯一的确诊依据,而目前临床上普遍反映核酸检测阳性率不够理想,故对病毒核酸检测的实验室条件、检验人员及检测过程中各个环节都有较高要求。本文着重阐述了新型冠状病毒核酸检测各个环节中的检测要点、最新的行业规范及共识内容,同时探讨临床检测中的疑难问题,以期为新型冠状病毒所致疾病的准确诊断提供理论依据。The number of pneumonia cases caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infections has risen rapidly since December 2019.Because the presence of viral nucleic acid in patients is the only basis for the diagnosis,and the positive rate of nucleic acid detection is generally not satisfactory in clinical practice,so there are high requirements for the laboratory conditions,inspectors of viral nucleic acid detection and other aspects of the detection process.This paper focuses on the current situation,the latest industry standards and the consensus contents of the SARS-CoV-2 nucleic acid testing,and probes into the difficult problems in clinical testing,so as to provide theoretical basis for the accurate diagnosis of the COVID-2019.

关 键 词：新型冠状病毒 新型冠状病毒肺炎 核酸检测 诊断 

分 类 号：R446.5[医药卫生—诊断学]