顶空毛细管气相色谱法测定迷迭香提取物中乙醇残留量  

Residual Ethanol of Rosemary Extraction Determined by Headspace Capillary Gas Chromatography

作　　者：李恬[1] 李琳 LI Tian;LI Lin(Yueyang Vocational and Technical College,Yueyang,Hunan 414000;National Institutes for Food and Drug Control,Beijing 100050)

机构地区：[1]岳阳职业技术学院,湖南岳阳414000 [2]中国食品药品检定研究院,北京100050

出　　处：《岳阳职业技术学院学报》2019年第6期77-79,共3页Journal of Yueyang Vocational and Technical College

基　　金：湖南省教育厅科学研究一般项目“洞庭湖区迷迭香温敏原位凝胶的制备及质量标准研究”（17C1623）

摘　　要：目的确定迷迭香提取物中乙醇残留量的测定方法,并测定其残留量。方法采用顶空毛细管气相色谱法,以水为溶剂,色谱柱为DB-WAX(30m×0.25mm×0.25μm),载气为氮气。进样口温度为220.0℃,FID检测器温度为250.0℃,柱温40℃;顶空进样,平衡温度为90℃,平衡时间为30min,分流比10:1。结果被测物能得到很好的分离,峰面积与浓度呈良好的线性关系,精密度和回收率良好。结论该法简单、灵敏、准确,适用于迷迭香提取物中乙醇残留量的检测。Objective:To determine the residual ethanol in Rosemary extraction.Methods:The headspace capillary gas chromatography is dissolved with water,the DB-WAX capillary column(30m*0.25mm*0.25μm)and nitrogen as carrier.The inlet and the detector temperature are controlled at 220℃ and 250℃ respectively,the column temperature is 40℃.The containers of head-space injector are in equilibrium at 90℃ for 30min and the splitting ratio was 10:1.Results:The samples are separated well in good linear condition that will be at accuracy and recovery rate.Conclusion:The method is simple,sensitive,accurate and that is suitable for the determination of residual ethanol in Rosemary extraction.

关 键 词：迷迭香 乙醇残留量 毛细管气相色谱 

分 类 号：O652.63[理学—分析化学]