苹果多胺氧化酶(PAO)基因家族鉴定与表达分析  

Identification and expression analysis of polyamine oxidase (PAO) gene family in apple

作　　者：秦玲[1] 张鑫[1] 荣春笑 莫传园 范露 闫婕 孟莹 张满让[1] QIN Ling;ZHANG Xin;RONG Chunxiao;MO Chuanyuan;FAN Lu;YAN Jie;MENG Ying;ZHANG Manrang(College of Horticulture,Northwest A&F University,Yangling 712100,China;Forestry Bureau of Luonan County,Luonan 726100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]洛南县林业局,陕西商洛726100

出　　处：《浙江农业学报》2020年第2期262-273,共12页Acta Agriculturae Zhejiangensis

基　　金：国家现代农业产业技术体系(CARS-27)

摘　　要：从金冠基因组数据库中,利用BLAST网站在苹果中鉴定得到8个编码多胺氧化酶(polyamine oxidase,PAO)的家族基因(命名为MdPAO1~8),对其进行理化性质、系统进化、蛋白结构、基因结构和启动子元件分析。结果表明,MdPAO蛋白的分子量为54.053~64.813 ku,等电点介于5.16~6.02。根据进化树分析,MdPAO被分为3个亚家族(Ⅰ、Ⅲ、Ⅳ),且同一亚家族的成员具有相似的蛋白结构、基因结构和蛋白保守基序分布。利用GEO数据库检测出8个MdPAO在不同组织器官中的表达具有明显差异。以苹果主栽品种长富2号为材料,利用实时荧光定量PCR检测分析得出8个MdPAO在不同组织中的表达情况,结果表明,MdPAO3、MdPAO4、MdPAO5/6和MdPAO8在花中有较高的表达水平;MdPAO1/2和MdPAO7在茎和果中有较高的表达水平。外源亚精胺处理后发现,除MdPAO3以外,其余MdPAO的转录水平显著提高,表明MdPAO在PA代谢途径中具有重要作用。From the genomic database of Golden Delicious,eight family genes encoding polyamine oxidase(PAO)(named as MdPAO 1-8)were identified in apples using the BLAST website for physicochemical properties,phylogenetic,protein structure,analysis of gene structure and promoter elements.The results showed that the molecular weight of MdPAO protein ranged from 54.053 to 64.813 ku,and the isoelectric point ranged from 5.16 to 6.02.According to the phylogenetic tree analysis,MdPAO is divided into three subfamilies(Ⅰ,Ⅲ,Ⅳ),and members of the same subfamily share similar protein structure,gene structure and protein conserved motif distribution.The expression of 8 MdPAO genes in different tissues and organs was significantly different using the GEO database.The expression of 8 MdPAO genes in different tissues was determined by real-time quantitative PCR using Nagafu No.2.The results showed that MdPAO3,MdPAO4,MdPAO5/6 and MdPAO8 were highly espressed in flowers.MdPAO1/2 and MdPAO7 had higher expression levels in stems and fruits.Exogenous spermidine treatment showed that the transcriptional level of MdPAO significantly increased except MdPAO3,indicating that MdPAO plays an important role in the PA metabolic pathway.

关 键 词：苹果 多胺氧化酶(PAO)基因家族 生物信息学 基因表达 

分 类 号：S661.1[农业科学—果树学]