黄芩对流感病毒FM1感染所致病毒性肺炎小鼠肺中炎性细胞因子蛋白与基因表达的影响  

Effect of Scutellariae Radix on expression of inflammatory cytokine protein and gene in lung of mice with viral pneumonia caused by influenza virus FM1 infection

作　　者：徐红日 李雅莉[3] 王成祥 刘国星 刘畅[1] 张莉莉 李青穆 李猛 曹鸿云 程淼 王红平 XU Hong-ri;LI Ya-li;WANG Cheng-xiang;LIU Guo-xing;LIU Chang;ZHANG Li-li;LI Qing-mu;LI Meng;CAO Hong-yun;CHENG Miao;WANG Hong-ping(Department of Emergency and Intensive Care Unit,the Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Sepsis,Beijing University of Traditional Chinese Medicine,Beijing 100029,China;Department of Emergency,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China;Department of Respiratory,Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi'an 710003,China;Department of Respiratory,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学第三附属医院急诊科和ICU,北京100029 [2]北京中医药大学脓毒症研究所,北京100029 [3]北京中医药大学东直门医院急诊科,北京100700 [4]陕西省中医医院肺病科,陕西西安710003 [5]北京中医药大学东直门医院呼吸科,北京100700

出　　处：《中国中药杂志》2019年第23期5166-5173,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81403371)

摘　　要：建立流感病毒亚洲甲型鼠肺适应株FM1感染所致病毒性肺炎小鼠模型,在FM1感染的不同时相,分别采用免疫组化法与RT-PCR法,动态观察清热解毒代表中药黄芩对流感病毒FM1感染所致小鼠病毒性肺炎模型靶器官肺中炎性细胞因子蛋白与基因表达的影响,阐明黄芩修复流感病毒所致肺炎靶器官免疫炎症损伤的部分作用机制。结果显示,黄芩在感染后第3~5天可降低肺组织中促炎性细胞因子TNF-α,IL-1,IL-6的蛋白与基因表达,在感染后第5天增加肺组织中抗炎性细胞因子IL-10和抗病毒因子IFN-γ的蛋白与基因表达。黄芩可能通过抑制促炎性细胞因子的过度释放,促进抗炎性细胞因子的表达,从而抑制机体全身炎症反应综合征,减轻流感病毒FM1感染所致靶器官肺的免疫炎症性病理损伤,促进肺组织炎性病变的修复。Mice models of viral pneumonia were induced by pulmonary adaptive strain FM1 of influenza A virus in Asian mice.RT-PCR and immunohistochemistry were used to dynamically observe the effect of Scutellariae Radix on the protein and gene expression of inflammatory cytokine in the lungs of the model mice infected by influenza virus FM1 at different phases.The partial mechanism of Scutellariae Radix in repairing the immune inflammatory damage of target organs of pneumonia caused by influenza virus was further explored.The results showed that Scutellariae Radix reduced protein and gene expression of proinflammatory cytokines tumor necrosis factor(TNF-α),interleukin IL-1,IL-6 in lung tissues from 3 rd to 5 th day after infection,and increased protein and gene expression of IL-10 and IFN-γin lung tissues on the 5 th day after infection.Scutellariae Radix may inhibit excessive release of pro-inflammatory cytokines and promote the expression of anti-inflammatory cytokines,thereby inhibiting the systemic inflammatory response syndrome,reducing the immunoinflammatory pathological damage of lung caused by influenza virus FM1 infection,and promoting lung repair of tissue inflammatory lesions.

关 键 词：黄芩 流感病毒FM1 肺炎 RT-PCR 免疫组化 细胞因子 

分 类 号：R285.5[医药卫生—中药学]