Neuroprotective effects of Suhexiang Wan on the in vitro and in vivo models of Parkinson’s disease  

作　　者：Liu Quanfeng Sang Heon Kim Yung-Wei Sung Sok Cheon Pak Wonwoong Lee Jongki Hong Jaehwan Jang Kyoung Sang Cho Songhee Jeon Byung-Soo Koo 

机构地区：[1]Department of Oriental Medicine,Dongguk University,Gyeogju,38066,Republic of Korea [2]Department of Oriental Neuropsychiatry,Graduate School of Oriental Medicine,Dongguk University,Gyeonggi-do 10326,Republic of Korea [3]College of Pharmacy,Kyung Hee University,Seoul 02447,Republic of Korea [4]School of Biomedical Sciences,Charles Sturt University,Bathurst,New South Wales,2795,Australia [5]Department of Biological Sciences,Konkuk University,Seoul 05029,Republic of Korea [6]Department of Biomedical Sciences,Center for Creative Biomedical Scientists at Chonnam National University,Gwangju 61469,Korea

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期800-808,共9页中医杂志（英文版）

基　　金：Supported by the Dongguk University Research Fund of 2014

摘　　要：OBJECTIVE:To examine the role of KSOP1009(a modified formulation of Suhexiang Wan essential oil)in an animal model of Parkinson's disease(PD)induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine(MPTP)injection.METHODS:Cell toxicity,apoptosis,and reactive oxygen species(ROS)levels were analyzed in the human neuroblastoma cell line SH-SY5 Y.After that,changes in animal behavior and tyrosine hydroxylase(TH)protein levels in the substantia nigra(SN)of MPTP-injected mice were examined.Three different doses of KSOP1009(30,100,and 300 mg/kg,n=8 for each group)were administered daily for 7 d before MPTP injection and 14 d after MPTP injection,totaling 21 d.RESULTS:MPP+,the active metabolite of MPTP,decreased the viability of SH-SY5 Y cells,whereas KSOP1009 alleviated MPP+-induced cytotoxicity.KSOP1009(10 and 50 mg/m L)reduced MPP+-induced ROS generation compared with the control group.Treatment with 1 m M MPP+increased the percentage of depolarized/live cells,whereas KSOP1009 intake at a dose of 10 mg/m L decreased the percentage of these cells.The mean latency to fall in the rotarod test was reduced in mice treated with MPTP compared with the control group.However,mice receiving three different doses of KSOP1009 performed better than MPTP-treated animals.MPTP-treated mice were more hesitant and took longer to traverse the balance beam than the control animals.In contrast,KSOP1009-treated mice performed significantly better than MPTPtreated mice.Furthermore,the KSOP1009-treated groups had a significantly higher number of TH-positive neurons in the lesioned SN and significantly higher expression of TH in the striatum than the MPTP-treated group.MPTP treatment strongly induced Jun-N-terminal kinase(JNK)activation,whereas KSOP1009 suppressed MPTP-induced JNK activation.In addition,KSOP1009 intake reversed the decrease in the phosphorylation levels of c AMP-response element-binding protein in the brain of MPTP-treated mice.KSOP1009 also restored the decrease in dopaminergic neurons and dopamine levels in the brain of MPTPOBJECTIVE: To examine the role of KSOP1009(a modified formulation of Suhexiang Wan essential oil) in an animal model of Parkinson’s disease(PD)induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine(MPTP) injection.METHODS: Cell toxicity, apoptosis, and reactive oxygen species(ROS) levels were analyzed in the human neuroblastoma cell line SH-SY5 Y. After that,changes in animal behavior and tyrosine hydroxylase(TH) protein levels in the substantia nigra(SN)of MPTP-injected mice were examined. Three different doses of KSOP1009(30, 100, and 300 mg/kg, n =8 for each group) were administered daily for 7 d before MPTP injection and 14 d after MPTP injection,totaling 21 d.RESULTS: MPP+, the active metabolite of MPTP, decreased the viability of SH-SY5 Y cells, whereas KSOP1009 alleviated MPP +-induced cytotoxicity.KSOP1009(10 and 50 mg/m L) reduced MPP +-induced ROS generation compared with the control group. Treatment with 1 m M MPP + increased the percentage of depolarized/live cells, whereas KSOP1009 intake at a dose of 10 mg/m L decreased the percentage of these cells. The mean latency to fall in the rotarod test was reduced in mice treated with MPTP compared with the control group. However, mice receiving three different doses of KSOP1009 performed better than MPTP-treated animals. MPTP-treated mice were more hesitant and took longer to traverse the balance beam than the control animals. In contrast, KSOP1009-treated mice performed significantly better than MPTPtreated mice. Furthermore, the KSOP1009-treated groups had a significantly higher number of TH-positive neurons in the lesioned SN and significantly higher expression of TH in the striatum than the MPTP-treated group. MPTP treatment strongly induced Jun-N-terminal kinase(JNK) activation,whereas KSOP1009 suppressed MPTP-induced JNK activation. In addition, KSOP1009 intake reversed the decrease in the phosphorylation levels of c AMP-response element-binding protein in the brain of MPTP-treated mice. KSOP1009 also restored the decrease in dopaminergic neurons

关 键 词：Parkinson disease 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine Mitochondria Reactive oxygen species Suhexiang Wan 

分 类 号：R285.5[医药卫生—中药学]