Liuwei Dihuang pill suppresses metastasis by regulating the wnt pathway and disrupting β-catenin/T cell factor interactions in a murine model of triple-negative breast cancer  

作　　者：Zheng Lixiang Zheng Qing Yu Zhipeng Wang Jian Ren Xiaoying Gong Yan Yang Xue Hunag Ping Weng Meizhi Liu Hongning Liu Haizhou 

机构地区：[1]School of Basic Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China [2]Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China [3]Department of Gastroenterology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China [4]Teaching and Research Office of Internal Medicine of Traditional Chinese Medicine,Department of Medicine,Jiangxi College of Traditional Chinese Medicine,Fuzhou 340000,China [5]School of Humanities,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期826-832,共7页中医杂志（英文版）

基　　金：Supported by the Chinese National Natural Science Foundation(No.81160531);Jiangxi Natural Science Foundation(No.20161BAB205223,20192ACBL21032);Scientific Research Fund of Jiangxi Provincial Education Department(No.GJJ180648)

摘　　要：OBJECTIVE:To investigate if the Liuwei Dihuang pill(LWDHP)can inhibit metastasis to the liver and lungs in mice bearing triple-negative breast cancer(TNBC),and the molecular mechanism underpinning this action.METHODS:Ninety-nine TNBC bearing-mice were distributed randomly to five groups:control(Con),paclitaxel(PTX),low-dose LWDHP(LLP,2.3 g·kg^-1·d^-1),middle-dose LWDHP(MLP,4.6 g·kg^-1·d^-1)and high-dose LWDHP(HLP,9.2 g·kg^-1·d^-1).The LWDHP were administered(p.o.)to the agonal stage.The morphology of BC cells was observed by hematoxylin&eosin staining.Expression of axin-2,β-catenin,T cell factor(TCF),cyclin-D1 and vascular endothelial growth factor(VEGF)was detected by western blotting or immunofluorescence.β-catenin/TCF-1 interaction was measured using a co-immunoprecipitation assay.RESULTS:After LWDHP treatment,metastasis of BC cells to the lungs and liver was inhibited,expression of axin-2 was increased,expression of TCF-1,β-catenin,cyclin-D1 and VEGF was decreased,andβ-catenin/TCF-1 interaction was disrupted.CONCLUSION:The LWDHP could inhibit metastasis of BC cells to the liver and lungs.The molecular mechanism underlying this action may be regulation of protein expression andβ-catenin/TCF-1 interactions in the Wnt pathway.OBJECTIVE: To investigate if the Liuwei Dihuang pill（LWDHP） can inhibit metastasis to the liver and lungs in mice bearing triple-negative breast cancer（TNBC）, and the molecular mechanism underpinning this action.METHODS: Ninety-nine TNBC bearing-mice were distributed randomly to five groups: control（Con）,paclitaxel（PTX）, low-dose LWDHP(LLP, 2.3 g·kg-1·d-1), middle-dose LWDHP(MLP, 4.6 g·kg-1·d-1)and high-dose LWDHP(HLP, 9.2 g·kg-1·d-1). The LWDHP were administered（p.o.） to the agonal stage. The morphology of BC cells was observed by hematoxylin & eosin staining. Expression of axin-2,β-catenin, T cell factor（TCF）, cyclin-D1 and vascular endothelial growth factor（VEGF） was detected by western blotting or immunofluorescence.β-catenin/TCF-1 interaction was measured using a co-immunoprecipitation assay.RESULTS: After LWDHP treatment, metastasis of BC cells to the lungs and liver was inhibited, expression of axin-2 was increased, expression of TCF-1,β-catenin, cyclin-D1 and VEGF was decreased, andβ-catenin/TCF-1 interaction was disrupted.CONCLUSION: The LWDHP could inhibit metastasis of BC cells to the liver and lungs. The molecular mechanism underlying this action may be regulation of protein expression and β-catenin/TCF-1 interactions in the Wnt pathway.

关 键 词：Triple negative breast neoplasms Neoplasm metastasis Beta catenin T cell transcription factor 1 Wnt signaling pathway Liuwei Dihuang pill 

分 类 号：R285.5[医药卫生—中药学]