Rongchang and Xifeng capsules suppress pentylenetetrazole-induced seizures and change rest/wake behavior in zebrafish larvae  

作　　者：Zhang Shuhui Xu Jia Wang Chao Feng Daofu Dai Liangti Kuang Xiangyu Zhu Shuyi Fang Yongchun Chen Dongyan He Xianghui Feng Xizeng 

机构地区：[1]State Key Laboratory of Medicinal Chemical Biology,The Key Laboratory of Bioactive Materials,Ministry of Education/College of Life Science,Nankai University,Tianjin 300071,China [2]Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation,Department of Histology and Embryology,School of Medicine,Nankai University,Tianjin 300071,China [3]The Institute of Robotics and Automatic Information Systems,Tianjin Key Laboratory of Intelligent Robotics,Nankai University,Tianjin 300071,China [4]Department of General Surgery,Tianjin Medical University General Hospital,Tianjin,300052,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期875-884,共10页中医杂志（英文版）

基　　金：Supported by the Chinese National Natural Science Foundation of China:In situ microscopic Analysis and manipulator for Life Science(No.61327802);the Chinese National Natural Science Foundation of China:Functional Nanoparticle Drug Vector for Zebrafish Development and Biocompatible Assessmen(No.81501589);the Chinese National Natural Science Foundation of China:Development of a cross-scale fast AFM system for life science(No.61127006);the Chinese National Natural Science Foundation of China:Study on the Method of Automatic Nano-manipulation for Cell-Oriented Accurate Displacement(No.61633012);the Tianjin Science Technology Research Funds of China:the function of Rab23 gene in zebrafish development(No.14JCQNJC09600);the National Basic Research Program of China:The Basic and Frontier of the New Topological Structure of the Molecular-based Functional Carbon Materials(No.2015CB856500)

摘　　要：OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules.Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic effects.The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day.Based on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling pathways.CONCLUSION:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules'effect on epilepsy.OBJECTIVE: To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS: We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules. Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS: Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500 μg/m L) and Xifeng(200 μg/m L) rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae, which indicated antiepileptic effects. The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day. Based on correlation between the actions of the two capsules and known compounds, we predicted that they might change rest/wake behaviors by affecting serotonin, GABAergic and histamine signaling pathways.CONCLUSION: The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules’ effect on epilepsy.

关 键 词：Zebrafish Larva ANTICONVULSANTS Rongchang capsule Xifeng capsule Rest/wake behavior 

分 类 号：R285.5[医药卫生—中药学]