Synthesis of core-shell structured Au@Bi2S3 nanorod and its application as DNA immobilization matrix for electrochemical biosensor construction  

作　　者：Feng Gao Juan Song Bin Zhang Hidekazu Tanaka Fei Gao Weiwei Qiu Qingxiang Wang 

机构地区：[1]Department of Chemistry and Environment Science,Minnan Normal University,Zhangzhou 363000,China [2]Department of Chemistry,Graduate School of Science and Engineering,Shimane University,Matsue,Shimane 690-8504,Japan [3]Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China

出　　处：《Chinese Chemical Letters》2020年第1期181-184,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21802064,21275127);Natural Science Foundation of Fujian Province,China (Nos.2018J01435,2017J01419);Foundation of Key Laboratory of Sensor Analysis of Tumor Marker,Ministry of Education,Qingdao University of Science and Technology

摘　　要：The core-shell structured Au@Bi2S3 nanorods have been prepared through direct in-situ growth of Bi2S3 at the surface of pre-synthesized gold nanorods.The product was characterized by X-ray diffraction,transmission electron microscopy and energy-dispersive X-ray spectroscopy.Then the obtained Au@Bi2S3 nanorods were coated onto glassy carbon electrode to act as a scaffold for fabrication of electrochemical DNA biosensor on the basis of the coordination of-NH2 modified on 5’-end of probe DNA and [e-mail] characterization assays demonstrate that the Au@Bi2S3 nanorods behave as an excellent electronic transport channel to promote the electron transfer kinetics and increase the effective surface area by their nanosize effect.The hybridization experiments reveal that the Au@Bi2S3 matrix-based DNA biosensor is capable of recognizing complementary DNA over a wide concentration ranging from 10 fmol/L to 1 nmol/L.The limit of detection was estimated to be 2 fmol/L(S/N=3).The biosensor also presents remarkable selectivity to distinguish fully complementa ry sequences from basemismatched and non-complementary ones,showing great promising in practical application.The core-shell structured Au@Bi2S3 nanorods have been prepared through direct in-situ growth of Bi2S3 at the surface of pre-synthesized gold nanorods.The product was characterized by X-ray diffraction,transmission electron microscopy and energy-dispersive X-ray spectroscopy.Then the obtained Au@Bi2S3 nanorods were coated onto glassy carbon electrode to act as a scaffold for fabrication of electrochemical DNA biosensor on the basis of the coordination of-NH2 modified on 5’-end of probe DNA and [e-mail] characterization assays demonstrate that the Au@Bi2S3 nanorods behave as an excellent electronic transport channel to promote the electron transfer kinetics and increase the effective surface area by their nanosize effect.The hybridization experiments reveal that the Au@Bi2S3 matrix-based DNA biosensor is capable of recognizing complementary DNA over a wide concentration ranging from 10 fmol/L to 1 nmol/L.The limit of detection was estimated to be 2 fmol/L（S/N=3）.The biosensor also presents remarkable selectivity to distinguish fully complementa ry sequences from basemismatched and non-complementary ones,showing great promising in practical application.

关 键 词：DNA biosensor Au@Bi2S3 nanorods Electrochemical impedance spectroscopy Core-shell structure Bi^3+-N bond Probe immobilization 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TB383.1[自动化与计算机技术—控制科学与工程]