26例稀土尘肺患者血清TNF-α、IL-1β、MCP-1、HYP检测结果分析  

Expression and significance of serum TNF-α,IL-1β,MCP-1 and HYP in patients with rare earth pneumoconiosis

作　　者：白宇超 王素华[1] 黄丽华[1] 乔瑞[1] 李怡博 吕佳玲 周灏 刘欣桐 BAI Yu-chao;WANG Su-hua;HUANG Li-hua;QIAO Rui;LI Yi-bo;LV Jia-ling;ZHOU Hao;LIU Xin-tong(College of Public Health,Baotou Medical College,Baotou,Inner Mongolia 014040,China)

机构地区：[1]包头医学院公共卫生学院,内蒙古包头014040

出　　处：《现代预防医学》2020年第5期947-950,共4页Modern Preventive Medicine

基　　金：国家自然科学基金项目(8186120351);国家自然科学基金项目(81760600);国家重点研发计划“精准医学研究”专项(2017YFC0907200);包头医学院科学研究基金项目(BYJJ-DF 201702);包头医学院博士科研启动基金项目(BSJJ201701)。

摘　　要：目的检测稀土尘肺患者血清中TNF-α、IL-1β、MCP-1、HYP的含量,探讨稀土尘肺早期诊断的敏感性指标。方法选择某稀土冶炼厂26名稀土尘肺患者作为稀土尘肺组,选择同期参加健康体检的不接触稀土粉尘的47人作为对照组,抽取空腹静脉血5 ml,采用ELISA法进行血清TNF-α、IL-1β、MCP-1、HYP表达水平检测。结果稀土尘肺患者血清中IL-1β、HYP水平高于对照组,TNF-α、MCP-1水平低于对照组(tTNF-α=-2.199,tIL-1β=2.884,tMCP-1=-3.280,tHYP=5.014,P<0.05);在稀土尘肺组中,IL-1β、MCP-1随着年龄的升高,表达水平逐渐上升(tIL-1β=-2.746,tMCP-1=2.873,P<0.05);吸烟组患者血清中IL-1β的表达水平高于非吸烟组(tIL-1β=-2.336,P<0.05),其余三项指标的差异无统计学意义;HYP随着稀土尘肺期别的升高,表达水平逐渐上升(tHYP=3.439,P<0.05);工龄在<12年组患者血清中IL-1β、MCP-1、HYP表达水平低于工龄在12~年组(tIL-1β=2.910,tMCP-1=2.859,tHYP=2.751,P<0.05);HYP表达水平在排除年龄、吸烟、期别三个混杂因素后,与工龄呈正相关(tHYP=2.257,P<0.05)。结论TNF-α、IL-1β、MCP-1、HYP在稀土尘肺患者血清中差异表达,对稀土尘肺的病情发展有重要意义,HYP可作为早期辅助诊断稀土尘肺的敏感性指标。Objective To measure the contents of serum TNF-α,IL-1 p,MCP-l and HYP in patients with rare earth pneumoconiosis,and to discuss the sensitive index of early auxiliary diagnosis of rare earth pneumoconiosis.Methods 26 patients diagnosed with rare earth pneumoconiosis in a city in recent three years were selected as the rare earth pneumoconiosis group,and 47 people who took part in physical examination at the same time were selected as the control group.5mL of fasting venous blood was collected and the expression of TNF-α,IL-1β and MCP-1,HYP in serum was detected by Enzyme-Linked Immunosorbent Assay(ELISA).Results The serum levels of IL-1β and HYP in patients with rare earth pneumoconiosis were higher than those in the control group,while the levels of TNF-a and MCP-1 in the patients with rare earth pneumoconiosis were lower than those in the control group(P<0.05).In rare earth pneumoconiosis group,the expression level of IL-1β and MCP-1 increased gradually with the increase of age(P<0.05).The expression level of IL-1β in serum of smoking group was higher than that of non-smoking group(P<0.05),but there was no significant difference among the other three indexes.The expression level of HYP increased gradually with the increase of rare earth pneumoconiosis stage(P<0.05).The expressions of IL-1β and MCP-1,HYP in serum of patients with length of service<12 years was significantly lower than those of patients with 12 years of service(P<0.05).The expression level of HYP was positively correlated with length of service after excluding age,smoking and age(P<0.05).Conclusion The differential expression of TNF-α,IL-1β and MCP-1,HYP in serum of rare earth pneumoconiosis patients is of great significance for the development of rare earth pneumoconiosis.IL-1βand HYP can be used as sensitive indexes for early diagnosis of rare earth pneumoconiosis.

关 键 词：稀土尘肺 肿瘤坏死因子-α 白细胞介素-1β 单核趋化蛋白-1 羟脯氨酸 

分 类 号：R135.2[医药卫生—劳动卫生]