荧光光谱法研究头孢克洛与牛血红蛋白的相互反应机理  

Mechanism of the Reaction Between Cefaclor and Bovine Hemoglobim by Fluorescence Spectrometry

作　　者：张彦青[1] 刘保生[2] ZHANG Yanqing;LIU Baosheng(Department of Applied Chemistry,Hengshui University,Hengshui 053000,China;College of Chem istry&Environmental Science,Hebei University,Baoding 071002,China)

机构地区：[1]衡水学院应用化学系,衡水053000 [2]河北大学化学与环境科学学院,保定071002

出　　处：《理化检验（化学分册）》2019年第12期1379-1383,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(21375032)

摘　　要：在pH 7.40的三(羟甲基)氨基甲烷缓冲溶液中,用荧光猝灭法和同步荧光法分别研究了不同温度(293,303,310K)条件下头孢克洛(CFC)与牛血红蛋白(BHB)的相互作用机理。结果表明:随着CFC浓度的增加,BHB的荧光强度和同步荧光强度均依次下降,说明两者之间形成配合物,CFC对BHB的荧光发生静态猝灭作用。在此配合反应过程中只有色氨酸参加反应,其结合位点数n均约为1。根据对该反应的热力学参数(ΔH、ΔS及ΔG)分析,说明两者之间的作用力主要是静电引力。试验结果表明:CFC的药物结合力随温度升高而减小,如从293K升至310K时,药物结合力由94.08%下降至93.14%,但其数值很大,表明CFC与BHB的结合使BHB能有效地将CFC传输到病灶部位;CFC与蛋白的结合率随温度上升基本不变(0.577%~0.571%)且数值很小,表明CFC与BHB的结合基本不影响BHB的游离浓度,即不影响其传输氧的功能。The mechanism of the reaction between cefaclor(CFC)and bovine hemoglobin(BHB)in a buffer solution of Tris-HCl(pH 7.40)was studied by fluorescence quenching and synchronous fluorescence spectrometry at different temperation conditions of 293 K,303 Kand 310 K.It was shown by the results that the fluorescence intensities obtained from both the fluorometry and the synchronous fluorometry were decreased regularly as the concentrations of CFC were increased,indicating that a coordinative complex was formed between CFC and BHB and that CFC showed a static quenching effect on the fluorescence of BHB.Only tryptophane radical of BHB was found to take part in the complexation reaction,and the number of binding site was found to be about one.Base on the analytical study of the thermodynamic parameters ofΔH,ΔS andΔG,it was found that electrostatic action was the main force of the reaction.As shown by the expermental results,values of drug binding rate(wQ)of CFC were decreased with raise of reaction temperature,e.g.,as the temperature raised from 293 Kto 310 K,values of wQ decreased from 94.08% to 93.14%,with the values quite large.It meant that the binding of CFC with BHB,made it possible for BHB to deliver CFC effectively to the focus of infection.It was also shown that values of protein binding rate(wB)were kept almost unchanged with the raise of reaction temperatures(wB0.577% to 0.571%),with the values quite small.It meant that the free concentration of BHB was not affected by the combination of CFC with BHB,i.e.,the oxygen conveying function of BHB was kept in normal state.

关 键 词：荧光光谱法 头孢克洛 牛血红蛋白 相互反应机理 

分 类 号：O657.3[理学—分析化学]