microRNA125a对缺血-再灌注心肌组织中巨噬细胞极化的影响  

Effect of microRNA125a on macrophage polarization during myocardial ischemia reperfusion

作　　者：郭冬娜 冀兵[1] 耿丽娟 李琼洁[1] 王云俊[1] Guo Dongna;Ji Bing;Geng Lijuan;Li Qiongjie;Wang Yunjun(Department of Emergency Medince,The First Hospital of Shanxi Medical University,Taiyuan 030001 China)

机构地区：[1]山西医科大学附属第一医院急诊医学中心,太原030001

出　　处：《中华急诊医学杂志》2020年第2期243-246,共4页Chinese Journal of Emergency Medicine

摘　　要：目的探讨microRNA125a在心肌缺血-再灌注中浸润巨噬细胞的表达,初步分析microRNA125a对心肌缺血-再灌注中浸润巨噬细胞极化的影响。方法建立大鼠心肌缺血-再灌注模型,取心肌损伤组织,分离浸润巨噬细胞,提取RNA。Qrt-PCR方法首先检测巨噬细胞中M1和M2标志分子,评估浸润巨噬细胞的类型。随后检测microRNA125a在浸润巨噬细胞中的表达水平,最后通过干预microRNA125a在巨噬细胞中的表达,进一步阐明microRNA125a对巨噬细胞极化的影响。结果缺血-再灌注心肌组织中巨噬细胞高表达Ml标志分子(TNF-α和IL-8),呈现Ml型巨噬细胞。同时microRNA125a在此巨噬细胞中也高表达。体外干预巨噬细胞中microRNA125a可以影响巨噬细胞的极化方向和水平。结论缺血-再灌注心肌组织中的巨噬细胞呈M1型,microRNA125a在此巨噬细胞中高表达并促进了其向Ml型偏移。Objective To investigate the expression of microRNA125a in infiltrating macrophages during myocardial ischemia reperfusion,and to analyze the effect of microRNA125a on the polarization of infiltrating macrophages during myocardial ischemia reperfusion.Methods The rat model of myocardial ischemia-reperfusion was established.Myocardial injury tissues were taken,infiltrated macrophages were isolated,and RNA was extracted.qRT-PCR was used to detect M1 and M2 markers in macrophages and evaluate the types of infiltrated macrophages.Subsequently the expression level of microRNA125a in infiltrating macrophages was detected.Finally,the effect of microRNA125a on macrophage polarization was further elucidated.Results Macrophages in myocardium of ischemia reperfusion showed high expression of M1 markers(TNF-alpha and IL-8),presenting M1 macrophages.At the same time,microRNA125a was also highly expressed in this macrophage.Knockdown or overexpression of microRNA125a in macrophages in vitro can affect the macrophages polarization.Conclusions Macrophages in myocardial ischemia reperfusion are M1 type,and microRNA125a is highly expressed in this macrophage and promotes its migration to M1 type.

关 键 词：microRNA125a 心肌缺血-再灌注 巨噬细胞 

分 类 号：R542.2[医药卫生—心血管疾病]