阿加曲班对大鼠脊髓损伤的修复作用  

Role of argatroban in repair of spinal cord injury in rats

作　　者：赵晨曦 樊保佑 李文祥 王旭[1] 庞翼林 姚雪[1] 冯世庆[1] Zhao Chenxi;Fan Baoyou;Li Wenxiang;Wang Xu;Pang Yilin;Yao Xue;Feng Shiqing(Department of Orthopedics,Tianjin Medical University General Hospital,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院骨科,300052

出　　处：《中华创伤杂志》2020年第1期86-92,共7页Chinese Journal of Trauma

基　　金：国家自然科学基金(81620108018,81672171)。

摘　　要：目的探讨阿加曲班对大鼠脊髓损伤的修复作用。方法选取54只雌性Wistar大鼠,使用脊髓损伤打击器制备大鼠脊髓挫伤模型。按随机数字表法分为假手术组(仅咬除T10椎板)、脊髓损伤组和脊髓损伤+阿加曲班组(阿加曲班组),每组18只。分别在造模前及伤后7,14,21,28,35,42 d利用BBB评分和斜板试验对大鼠后肢运动功能进行评估;伤后42 d检测各组大鼠后肢感觉诱发电位(SEP)和运动诱发电位(MEP),运用HE染色观察损伤区域脊髓组织形态学变化并比较空洞大小。结果伤后7 d,阿加曲班组BBB评分[(3.7±0.5)分]及斜板试验[(28.0±2.6)°]较脊髓损伤组[(3.3±0.5)分、(24.3±1.9)°]有明显改善(P<0.05)。伤后42 d,阿加曲班组BBB评分[(13.0±0.8)分]及斜板试验[(50.7±2.7)°]达到峰值,且明显优于脊髓损伤组[(9.7±1.3)分、(40.5±2.7)°](P<0.05);而阿加曲班组伤后BBB评分及斜板试验均低于假手术组[(21.0±0.0)分、(60.0±0.0)°](P<0.05)。伤后42 d,阿加曲班组SEP潜伏期[(25.0±0.9)ms]较脊髓损伤组[(31.5±1.9)ms]明显缩短,波幅[(2.1±0.1)μV]较脊髓损伤组[(0.5±0.1)μV]明显升高(P均<0.05);而潜伏期和波幅较假手术组[(19.5±1.0)ms、(2.8±0.1)μV]延长或降低(P均<0.05)。阿加曲班组MEP潜伏期[(11.5±1.0)ms]较脊髓损伤组[(17.5±1.1)ms]明显缩短,波幅[(4.8±0.8)μV]较脊髓损伤组[(2.8±0.7)μV]明显升高(P均<0.05);而潜伏期和波幅较假手术组[(7.5±1.0)ms、(7.5±1.0)μV]延长或降低(P均<0.05)。HE染色结果显示,阿加曲班组损伤中心区域空洞面积[(0.35±0.04)mm2]较脊髓损伤组[(0.71±0.05)mm2]明显减小(P<0.05)。结论阿加曲班可修复大鼠脊髓损伤组织,抑制脊髓空洞形成,促进其后肢感觉和运动功能恢复。Objective To investigate the effect of argatroban in repair of spinal cord injury in rats.Methods A total of 54 female Wistar rats were selected and divided into three groups according to the random number table:sham group,injury group and Argatroban group,with 18 rats in each group.The sham group only took the T10lamina;the injury group used the spinal cord injury device to make the rat spinal cord injury model;the Argatroban group received Argatroban treatment after spinal cord injury.The recovery of hindlimb motor function was evaluated by BBB score and clined plate test before injury and 7,14,21,28,35 and 42 days after injury.The sensory evoked potentials(SEP)and motor evoked potentials(MEP)were detected 42 days after operation.HE staining was used to compare the size of the cavity in the local region 42 days after injury.Results At day 7 after injury,the BBB score was(3.7±0.5)points and the inclined plane test was(28.0±2.6)°in the Argatroban group,which were better than those in the injury group[(3.3±0.5)points,(24.3±1.9)°](P<0.05).At day 42 after injury,the BBB score was(13.0±0.8)points and inclined plane test was(50.7±2.7)°in the Argatroban group,which were significantly better than those in the injury group[(9.7±1.3)points,(40.5±2.7)°](P<0.05).But all the above values in the Argatroban group were significantly lower than those in the sham group[(21.0±0.0)points,(60.0±0.0)°](P<0.05).At day 42 after operation,the SEP latency[(25.0±0.9)ms]in the Argatroban group was significantly shorter than that in the injury group[(31.5±1.9)ms];the amplitude[(2.1±0.1)μV]in the Argatroban group was lower than that in the injury group[(0.5±0.1)μV](P<0.05).The MEP latency[(11.5±1.0)ms]in the Argatroban group was significantly shorter than that in the injury group[(17.5±1.1)ms],and the amplitude[(4.8±0.8)μV]in the Argatroban group was lower than that in the injury group[(2.8±0.7)μV](P<0.05).And the SEP or MEP latency and amplitude in the Argatroban group showed significant differences compared to th

关 键 词：脊髓损伤 阿加曲班 炎症 凝血酶 

分 类 号：R651[医药卫生—外科学]