解脲支原体感染对表征男性精子质量关键参数影响的全面分析  

A comprehensive analysis of the effects of ureaplasma urealyticum infection on the key parameters of male sperm quality

作　　者：宋伟杰 刘心怡 刘建业[1,3] 刘祥虎[1] 伍拓 何乐业[1,3] Song Weijie;Liu Xinyi;Liu Jianye;Liu Xianghu;Wu Tuo;He Leye(Department of Urology,The Third Xiangya Hospital,Central South University,Changsha,Hunan 410013,China;Department of Obstetrics and Gynecology,The Third Xiangya Hospital,Central South University,Changsha,Hunan 410013,China;Institute of Prostate Disease,Central South University,Changsha,Hunan 10013,China)

机构地区：[1]中南大学湘雅三医院泌尿外科,湖南长沙410013 [2]中南大学湘雅三医院妇产科,湖南长沙410013 [3]中南大学前列腺疾病研究所,湖南长沙410013

出　　处：《中国男科学杂志》2019年第6期32-35,共4页Chinese Journal of Andrology

摘　　要：目的掌握解脲支原体感染对表征男性精子质量全部关键参数的影响情况,使相关疾病的临床诊治更有针对性。方法收集自2018年5月至2019年7月因男性生育问题前来我院门诊就诊患者的精液样本,利用北昂(BEION S3-2)全自动精子质量分析仪行精液常规检查,获取表征精子质量的各项参数,利用支原体分离鉴定试剂盒对所收集的精液进行解脲支原体培养。采用Mann-Whitney U检验对解脲支原体培养阴性和阳性样本的精液常规检查结果进行了统计学分析,分析结果用中位数(四分位数间距)表述。结果精液解脲支原体培养阴性组(对照组)共188例,年龄中位数为30.0(28.0~32.0)岁;阳性组(病例组)共188例,年龄中位数为30.0(28.0~34.0)岁,年龄差异无统计学意义(P=0.417)。两组患者的精液pH值(P=0.417)和排精量(P=0.558)差异也没有统计学意义,而两组的精液液化状态分布差异具有统计学意义(P<0.001)。此外,两组的其他多项精液参数差异也都具有统计学意义(P均<0.001)且阳性组的中位数均小于阴性组,这些参数包括:前向运动(PR)精子百分率、精子总活力、正常形态精子(%)、精子浓度及精子存活率。结论解脲支原体感染影响了男性精子质量,可认为是导致男性不孕不育的重要因素之一。Objective To investigate the effects of ureaplasma urealyticum infection on the key parameters of male sperm quality for accurate diagnosis and treatment of related diseases.Methods From May 2018 to July 2019,semen samples of outpatient male patients with fertility were collected.Routine semen examination was performed using BEION S3-2 automatic sperm quality analyzer to obtain various parameters for characterizing sperm quality,and the collected semen was cultured for detection of ureaplasma urealyticum using the mycoplasma separation and identification kit.The Mann-Whitney U test was used to analyze the parameters of conventional semen examination of negative and positive samples of ureaplasma urealyticum culture,and the results were expressed by median(interquartile range).Results There were 188 negative cases of ureaplasma urealyticum culture,the median age was 30.0(28.0-32.0)years old;188 cases in the positive group,the median age was 30.0(28.0-34.0)years old,and the age difference was not statistically significant(P=0.417).There were no statistically significant differences in semen PH(P=0.417)and semen discharge(P=0.558)between the two groups,but the difference in semen liquefaction status was statistically significant(P<0.001).Moreover,the differences in other semen parameters of the two groups were also statistically significant,and the median of the positive group were smaller than those of the negative group.These parameters included semen forward motion(PR)(%)(P<0.001),total semen activity(PR+NP)(%)(P<0.001),normal morphology sperm(%)(P<0.001),sperm concentration(106/m L)(P<0.001)and sperm survival rate(%)(P<0.001).Conclusion Ureaplasma urealyticum infection decreases the quality of male sperm,and probably is one of the important factors for occurrence of male infertility.

关 键 词：解脲支原体 精子分析 男性生育 感染 

分 类 号：R698.2[医药卫生—泌尿科学] R691.3[医药卫生—外科学]