12C6+离子束辐照黄花蒿干种子当代生物学效应  

Biological Effects of 12C6+ Ion Beams on Dry Seeds of Artemisia annua L.

作　　者：石紫薇 李雪虎[2] 梁妍 姜婷婷[2] 梁剑平[2] 贾宁[1] SHI Ziwei;LI Xuehu;LIANG Yan;JIANG Tingting;LIANG Jianping;JIA Ning(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China;School of Pharmacy,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国科学院近代物理研究所,兰州730000 [3]兰州大学药学院,兰州730000

出　　处：《原子核物理评论》2019年第4期499-504,共6页Nuclear Physics Review

基　　金：“十三五”国家重大科技专项重大新药创制项目(2017ZX09101002-003-001)~~

摘　　要：利用12C^6+重离子束对黄花蒿干种子辐照后,利用发芽率、根长、下胚轴长和株高4个指标研究不同剂量12C^6+离子束辐照对黄花蒿的生物学效应。结果表明,随着辐照剂量的增加,黄花蒿的存活率和根长都呈现逐渐降低的趋势,而下胚轴长先上升后下降,株高总体呈现下降的趋势,但在60和90 Gy同时存在矮化与增高的植株。利用SRAP(sequence related amplified polymorphism)技术对辐照后M1代的黄花蒿植株进行研究,结果表明对照组和处理组之间的差异体现为特异性条带的变化。综合分析表明,12C^6+的重离子辐照黄花蒿干种子可以产生显著的当代损伤效应,同时引起植物DNA的多态性变化。To study the biological effects of 12C^6+ion beams on dry seeds of Artemisia annua L,there are four indicators:survival rate,root length,hypocotyls length,plant height.The results showed that survival rate and root length decreased with increasing doses.While the hypocotyl length first increased and then decreased,the plant height showed a trend of overall decrease,but the plant height increased and decreased at 60 and 90 Gy.After studying the application of M1 generation after irradiation of artemisinin SRAP(sequence related amplified polymorphism)technique,the results showed that the differences between control group and treatment group are embodied at the change of specific bands.All the results indicated that M1 seedings of Artemisia annua L.irradiated by 12C^6+ion has obvious biological effects on both the plant developments and genomic DNA variations.

关 键 词：12C^6+离子束 辐照 黄花蒿 生物学效应 SRAP 

分 类 号：Q691[生物学—生物物理学]