海拔和冰核细菌对茴香薄翅野螟过冷却点的影响  

Effects of altitude and ice nucleating active bacteria on the super-cooling point of Evergestis extimalis Scopli(Lepidoptera: Pyralidida)

作　　者：来有鹏[1] 李秋荣[1] 张贵[1] LAI You-Peng;LI Qiu-Rong;ZHANG Gui(Key Laboratory of Plateau Ecology and Agriculture,Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province,Qinghai Academy of Agricultural and Forestry Sciences,Xining 810016,China)

机构地区：[1]省部共建三江源生态与高原农牧业国家重点实验室青海省农业有害生物综合治理重点实验室青海省农林科学院

出　　处：《应用昆虫学报》2019年第6期1347-1352,共6页Chinese Journal of Applied Entomology

基　　金：青海省科技厅重大专项(2019NKA11)

摘　　要：【目的】为了明确高原环境条件下海拔和冰核细菌对茴香薄翅野螟EvergestisextimalisScopli耐寒性的影响。【方法】采用模拟大田法,研究了茴香薄翅野螟过冷点和冰点的变化动态。【结果】2014年2月乐都(LD,海拔:1 900 m)、西宁(XN,海拔:2 300 m)和湟源(HY,海拔:3 010 m)茴香薄翅野螟的过冷却点分别为-12.30、-13.39和-14.56℃,且过冷却点和冰点的变化与当地气温的变化一致。经冰核细菌处理后能提高茴香薄翅野螟的过冷却点。2014年2月,冰核细菌1-1(丁香假单胞菌,Pseudomonas syringae)、冰核细菌2-1(丁香假单胞菌,Pseudomonas syringae)、冰核细菌3-1(欧文氏菌,Erwiniapyrifoliae)和对照处理后的过冷却点分别为-10.72、-13.73、-14.04和-14.39℃。经冰核细菌1-1、冰核细菌2-1、冰核细菌3-1和对照处理后,茴香薄翅野螟越冬幼虫死亡率分别为29.09%、36.17%、33.16%和27.44%。【结论】随着海拔的增加,茴香薄翅野螟的过冷却点不断降低;经冰核细菌处理后,茴香薄翅野螟的过冷却点显著降低,且能提高茴香薄翅野螟越冬幼虫的死亡率。[Objectives] To understand how cold tolerance is influenced by altitude and ice nucleating active bacteria(INA)in high altitude environments. 【Methods】 Field conditions were simulated in a laboratory to study the effects of varying the super-cooling point(SCP) and freezing point(FP) of Evergestis extimalis.【Results】 Super cooling point declined gradually with altitude. For example, the SCP of E. extimalis from Le Du(1 900 m ASL), Xing Ning(2 300 m ASL) and Huang Yuang(3010 m ASL) were ﹣12.30,﹣13.39 and﹣14.56 ℃, respectively. Variation of SCP and freezing point were correlated with local temperature. The SCP of E. extimalis increased after INA treatment. E. extimalis in the following treatment groups;INA1-1(Pseudomonas syringae), INA2-1(P. syringae), INA3-1(Erwinia pyrifoliae) and the control, had SCPs of-10.72,-13.73,-14.04 and-14.39 ℃, respectively in Feb. 2014. Mortality in the INA1-1, INA2-1, INA3-1 treatment groups and the control were 29.09%、36.17%、33.16% and 27.44%, respectively on Nov. 2013.【Conclusion】 The SCP of E. extimalis decreased with increasing altitude. INA treatment dramatically reduced mortality and increased the SCP of E. extimalis.

关 键 词：海拔 冰核细菌 茴香薄翅野螟 过冷却点 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]