旱黄瓜未授粉子房雌核诱导技术体系研究  

Female Nucleus Development Induction Technique System in Cucumber Non-pollination Ovary

作　　者：郭晓雨 宋晓飞[1] 李晓丽[1] 孙成振[1] 冯志红[1] 闫立英[1] GUO Xiao-yu;SONG Xiao-fei;LI Xiao-li(College of Horticulture Science and Technology,Hebei Normal University of Science&Technology,Qinhuangdao,Hebei 066004)

机构地区：[1]河北科技师范学院园艺科技学院

出　　处：《安徽农业科学》2020年第5期51-54,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省科技计划项目(16226308D-4);河北省高等学校科学技术研究项目(BJ2018002);河北省现代农业产业技术体系创新团队建设项目(HBCT2018030209)

摘　　要：以4种不同基因型旱黄瓜的未授粉子房为试材,研究了基因型、子房不同发育时期及采样时间、不同质量浓度的TDZ、温度对雌核诱导的影响,旨在探究旱黄瓜未授粉子房诱导雌核发育的技术体系。结果表明,不同基因型对雌核发育的影响差异显著,“1503”的雌核启动率最高,为77.22%;开花前1 d的样品最优,此时1503雌核启动率为95.56%;随TDZ质量浓度上升,雌核启动率呈先上升后下降趋势,其中0.08 mg/L TDZ处理的1503雌核启动率最高,达91.6%;35℃暗培养4 d的处理,雌核启动率最优,达78.33%。旱黄瓜未授粉子房培养最优雌核诱导技术体系:以开花前1 d未授粉子房为材料,在添加0.08 mg/L TDZ的诱导培养基上进行35℃暗培养4 d。该研究成功建立了旱黄瓜未授粉子房雌核诱导技术体系,为旱黄瓜遗传育种及基础理论研究提供技术支撑。The effects of genotype,ovary development stage and sampling time,TDZ treatment with different mass concentration,and temperature on esterium induction were studied in unpollinated ovary of cucumber.The results showed that there were significant differences in the effects of different genotypes on the development of the female nucleus.The sample was optimal 1 day before flowering,when the activation rate of the female nucleus of“1503”was 95.56%.With the increase of TDZ mass concentration,the activation rate of female nucleus increased first and then decreased,among which the activation rate of 1503 female nucleus treated with 0.08mg/L TDZ was the highest,up to 91.6%.After dark culture at 35℃for 4 days,the activation rate of female nucleus was the best,up to 78.33%.In this study,the optimal estrus induction technology system for the culture of unpollinated ovary of drought cucumber was as follows:the unpollinated ovary was taken as the material 1 day before flowering,and dark culture was conducted at 35℃ for 4 days on the induction medium supplemented with 0.08 mg/L TDZ.In this study,the technology system of ovum estrogen-nucleus induction in unpollinated ovary of drought cucumber was preliminarily explored,which provided technical support for the genetic breeding and basic theoretical research of drought cucumber.

关 键 词：旱黄瓜 未授粉子房 雌核启动率 

分 类 号：S642.2[农业科学—蔬菜学]