乌鲁木齐市外环境标本禽流感病毒监测情况及相关因素分析  

Surveillance of avian influenza virus in external environment specimens and analysis of its related factors in Urumqi

作　　者：韩志国[1] 薛娜[1] 樊旭成[1] 高枫[1] HAN Zhi-guo;XUE Na;FAN Xu-cheng;GAO Feng(Urumqi Center for Disease Control and Prevention,Urumqi,Xinjiang 830026,China)

机构地区：[1]乌鲁木齐市疾病预防控制中心

出　　处：《实用预防医学》2020年第2期135-137,共3页Practical Preventive Medicine

基　　金：乌鲁木齐市新发和重点传染病快速监测重点实验室(H171313001)

摘　　要：目的分析乌鲁木齐市外环境标本禽流感病毒阳性检出情况及相关因素,为进一步规范外禽环境标本的采样和检测流程及疫情防控提供依据。方法对2015年1月—2017年12月三年期间采集的909份禽类外环境标本,采用实时荧光定量PCR方法进行核酸检测,分析禽流感病毒阳性检出率及相关因素。结果在909份标本中,共检出禽流感阳性116份,阳性率为12.76%;阳性标本中,以H9亚型为主;不同监测场所和不同季度禽流感病毒阳性检出率差异有统计学意义(P<0.05),以城乡活禽市场和第一季度的阳性率最高,分别为16.45%(90/547)、22.33%(46/206);不同标本类型和不同的送检检测时间间隔的病毒阳性检出率差异无统计学意义(均P>0.05)。结论乌鲁木齐市外环境禽流感病毒阳性检出率偏高,存在禽流感流行风险,监测场所和监测季度是禽流感病毒阳性检出率相关因素。Objective To analyze the positive detection of avian influenza virus in external environmental specimens and its related factors in Urumqi,and to provide a basis for further standardization of sampling and detection process of external environmental specimens and epidemic prevention and control.Methods A total of 909 specimens were collected from poultry-related external environment between January 2015 and December 2017.Real-time fluorescence quantitative PCR was used to detect nucleic acid,and the positive detection rate of avian influenza virus and its related factors were analyzed.Results Among the 909 specimens,116 were positive for avian influenza virus,with the positive rate of 12.76%.Among the positive specimens,H9 subtype played a dominant role.The positive detection rate of avian influenza virus was significantly different in different monitoring sites and seasons(both P<0.05),and the positive detection rates in urban and rural live poultry markets(16.45%,90/547)and the first quarter(22.33%,46/206)were found to be the highest.No statistically significant differences were found in the positive rate of avian influenza virus among different types of specimens as well as in different time intervals between submission time and detection time(both P>0.05).Conclusions The positive detection rate of avian influenza virus in external environment in Urumqi city is high,and there is a risk for avian influenza virus epidemic.Monitoring sites and quarters are factors related to the positive detection rate of avian influenza virus.

关 键 词：外环境 禽流感病毒 阳性检出率 H9亚型 

分 类 号：R511.7[医药卫生—内科学]