反硝化-高效部分亚硝化-厌氧氨氧化工艺处理老龄垃圾渗滤液  

Nitrogen Removal from Mature Landfill Leachate via Denitrification-Partial Nitritation-ANAMMOX Based on a Zeolite Biological Aerated Filter

作　　者：陈小珍 汪晓军 Karasuta Chayangkun[1,2] 周松伟 钟中 陈振国 陈晓坤 CHEN Xiao-zhen;WANG Xiao-jun;Karasuta Chayangkun;ZHOU Song-wei;ZHONG Zhong;CHEN Zhen-guo;CHEN Xiao-kun(School of Environment and Energy,South China University of Technology,Guangzhou 510006,China;Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Cluster,Ministry of Education,Guangzhou 510006,China;School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510665,China;Hua An Biotech Co.,Ltd.,Foshan 528300,China)

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [3]华南理工大学化学与化工学院,广州510665 [4]佛山市化尔铵生物科技有限公司,佛山528300

出　　处：《环境科学》2020年第1期345-352,共8页Environmental Science

基　　金：2017年广东省应用型科技研发及重大科技成果转化专项(2017B020236004)

摘　　要：采用反硝化-沸石曝气生物滤池(ZBAF)部分亚硝化及氧氨氧化组合工艺处理老龄垃圾渗滤液,探究ZBAF部分亚硝化特性以及组合工艺的脱氮除碳性能.结果表明通过游离氨(FA)对亚硝酸盐氧化菌(NOB)的选择性抑制ZBAF可以实现老龄垃圾渗滤液稳定高效部分亚硝化,平均亚硝氮积累率(NAR)为93.8%亚硝氮产率(NPR)最高达1.659 kg·(m3·d-1;在进水中投加葡萄糖700mg·L-1后,当回流比为2.0 HRT为2.2 d时,由于反硝化与厌氧氨氧化的协同作用,组合工艺脱氮效果最佳,平均氨氮去除率(ARE)、总氮去除率(NRE)和总氮去除负荷(NRR)分别达97.2%、90.0%和0.585 kg·(m3·d)-1,平均COD去除率为45.3%其中厌氧氨氧化平均NRRANA为1.060 kg·(m3·d)-1最高达1.268 kg·(m3·d)-1.利用高通量测序技术分析各装置中的微生物群落结构.结果显示,反硝化细菌(Paracoccus和Comamonnas)、氨氧化细菌(AOB)(Nitrosomonas)和厌氧氨氧化菌(Candidatus Kuenenia和Candidatus Anammoxoglobus)分别为反硝化、ZBAF和厌氧氨氧化装置中的优势菌这与组合工艺稳定的脱氮性能相吻合.A combined process of denitrification-partial nitritation-ANAMMOX based on a zeolite biological aerated filter(ZBAF) was applied to treat mature landfill leachate.We investigate the partial nitritation characteristics of the ZBAF and the nitrogen removal performance as well as the carbon removal performance of the combined process.Results showed that,based on the selective inhibition of nitrite oxidizing bacteria(NOB) by free ammonia(FA),the ZBAF could successfully achieve stable and efficient partial nitrification of mature landfill leachate,with an average nitrite accumulation rate(NAR) of 93.8% and a maximum nitrite production rate(NPR) of 1.659 kg·(m3·d)-1.After adding 700 mg·L-1 glucose to the influent,due to the synergistic effect of denitrification and anammoxidation,the combined process achieved its best nitrogen removal performance at a reflux ratio of 2.0 and hydraulic retention time(HRT) of 2.2 days.The average ammonia removal efficiency(ARE),total nitrogen removal efficiency(NRE),total nitrogen removal loading rate(NRR),and average chemical oxygen demand(COD) removal efficiency were 97.2%,90.0%,0.585 kg·(m3·d)-1,and 45.3%,respectively.Furthermore,the NRR of the anaerobic ammonium oxidation(ANAMMOX) process(NRRANA) reached 1.268 kg·(m3·d)-1.High-throughput sequencing technology was used to analyze the microbial community structure in each device.Results showed that denitrifiers(Paracoccus and Comamonas),ammonia-oxidizing bacteria(AOB)(Nitrosomonas),and ANAMMOX bacteria(Candidatus Kuenenia and Candidatus Anammoxoglobus) were the dominant bacteria in the UASB,ZBAF,and ANAMMOX reactor,respectively,which corresponded to the stable nitrogen removal performance of the combined process.

关 键 词：老龄垃圾渗滤液 前置反硝化 部分亚硝化 厌氧氨氧化(ANAMMOX) 沸石曝气生物滤池 

分 类 号：X703.1[环境科学与工程—环境工程]