Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater  

作　　者：CUI Wenxiao MA Aijun HUANG Zhihui WANG Xin’an SUN Zhibin LIU Zhifeng ZHANG Wei YANG Jingkun ZHANG Jinsheng QU Jiangbo 

机构地区：[1]College of Fisheries and Life Science,Shanghai Ocean University,Ministry of Education,Shanghai 201306,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China [3]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding,Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Qingdao 266071,China [4]Yantai Tianyuan Aquatic Limited Corporation,Yantai 264003,China

出　　处：《Journal of Oceanology and Limnology》2020年第2期467-479,共13页海洋湖沼学报（英文）

基　　金：Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-47-G01);the Ao Shan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS04);the National Natural Science Foundation of China(No.41706168);the Agricultural Fine Breed Project of Shandong(No.2019LZGC013);the Basal Research Fund,Chinese Academy of Fishery Sciences(No.2016HY-JC0301);the Yantai Science and Technology Project(No.2018ZDCX021)

摘　　要：Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity.The kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5)and seawater(salinity 30)was sequenced and characterized.In silico analysis indicated that all unigenes had significant hits in seven databases.The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity.Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity.Moreover,we analyzed and predicted the mechanisms of canonical signaling transduction.Biological processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the turbot.In addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out.The results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity.The kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5) and seawater(salinity 30) was sequenced and characterized.In silico analysis indicated that all unigenes had significant hits in seven databases.The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity.Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity.Moreover,we analyzed and predicted the mechanisms of canonical signaling transduction.Biological processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the turbot.In addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out.The results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.

关 键 词：osmoregulation mechanisms Scophthalmus maximus hypo-saline signal transduction cell volume regulation gene expression 

分 类 号：S917.4[农业科学—水产科学]