Heritability of resistance-related gene expression traits and their correlation with body size of clam Meretrix petechialis  

作　　者：JIANG Fengjuan YUE Xin ZHANG Shujing YU Jiajia WANG Rui LIU Baozhong WANG Hongxia 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266000,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2020年第2期571-578,共8页海洋湖沼学报（英文）

基　　金：Supported by the China Agriculture Research System(No.CARS-49);the National Natural Science Foundation of China(No.31772845);the Science and Technology Service Network Initiative of the Chinese Academy of Sciences(No.KFJ-STS-ZDTP-049);the Foundation of Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource(No.J2018001)

摘　　要：Gene expression variation can be considered as a phenotype,and it plays an important role in both acclimation and adaption.However,genetic variation of gene expression received much less attention than traditional commercial traits in aquaculture.To estimate the genetic variation and heritability of gene transcription in clam Meretrix petechialis,five Vibrio resistance-related genes were selected for gene expression analysis in the digestive gland,and an animal linear model was used to analyze data from quantitative real-time PCR(qRT-PCR).Among the five genes,BIRC7 showed significant additive genetic variations,the heritability of this gene of 12-month-and 15-month-old clams were 0.84±0.32 and 0.91±0.34,respectively.The heritability of other four genes(Bax,NFIL3,Big-Def,and CTL9)expression were low-tomoderate but not significantly expressed.Additionally,no significant phenotypic and genetic correlations between the BIRC7 transcription trait and body size were detected.This study highlights that certain gene expression variation is heritable and provides a reference for indirect selection of M.petechialis with high Vibrio resistance.Gene expression variation can be considered as a phenotype,and it plays an important role in both acclimation and adaption.However,genetic variation of gene expression received much less attention than traditional commercial traits in aquaculture.To estimate the genetic variation and heritability of gene transcription in clam Meretrix petechialis,five Vibrio resistance-related genes were selected for gene expression analysis in the digestive gland,and an animal linear model was used to analyze data from quantitative real-time PCR(qRT-PCR).Among the five genes,BIRC7 showed significant additive genetic variations,the heritability of this gene of 12-month-and 15-month-old clams were 0.84±0.32 and 0.91±0.34,respectively.The heritability of other four genes(Bax,NFIL3,Big-Def,and CTL9) expression were low-tomoderate but not significantly expressed.Additionally,no significant phenotypic and genetic correlations between the BIRC7 transcription trait and body size were detected.This study highlights that certain gene expression variation is heritable and provides a reference for indirect selection of M.petechialis with high Vibrio resistance.

关 键 词：Meretrix petechialis transcription trait body size HERITABILITY genetic correlation 

分 类 号：S917.4[农业科学—水产科学]