一测多评法同时测定乳癖消片中5种指标性成分的研究  

Determination of Five Index Components in Rupixiao Tablets by Quantitative Analysis of Multi-components by Single Marker

作　　者：邓艳梅 徐建 Deng Yanmei;Xu Jian(Chuzhou Institute for Food and Drug Control,Anhui Chuzhou 239000,China)

机构地区：[1]滁州市食品药品检验中心

出　　处：《中国药师》2020年第1期179-182,共4页China Pharmacist

摘　　要：目的:建立一测多评(QAMS)法来测定乳癖消片中5种指标性成分的含量。方法:采用HPLC法,以岛津Inertsil ODS-3(250 mm×4.6 mm,5μm)为色谱柱;流动相为乙腈-水溶液,梯度洗脱;流速:1.0 ml·min^-1;检测波长为可变波长(0 min,230 nm;18 min,203 nm;26.5 min,275 nm;35 min,203 nm);柱温:30℃;进样量:10μl。以连翘苷为参照物建立5种指标性成分之间的相对校正因子,分别采用外标法和一测多评法测定乳癖消片中的5种指标性成分的含量,并对两种方法所得结果进行比较。结果:在一定的线性范围内,连翘苷与芍药苷、人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的相对校正因子为0.411,2.277,3.744,2.776,且在不同试验条件下重现性良好。一测多评法的计算值与外标法测定值间差异无统计学意义(P>0.05)。结论:所建立的QAMS法可用于测定乳癖消片中5种指标性成分的含量。Objective:To develop a quantitative analysis method for five index components in Rupixiao tablets by multi-components by single marker(QAMS).Methods:An HPLC method was used,and the column was Shimadzu Inertsil ODS-3(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-water with gradient elution at the flow rate of 1.0 ml·min^-1,the detection wavelength was switched as follows:0 min,230 nm;18 min,203 nm;26.5 min,275 nm;35 min,203 nm.The column temperature was 30℃with the injection volume of 10μl.With phillyrin as the internal standard,the relative correction factors among the five index components were established.The five index contents were determined by an external standard method and QAMS,respectively.The results of the two methods were compared to confirm the practicality and stability of QAMS.Results:The RCF of paeoniflorin,notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 to phillyrin was 0.411,2.277,3.744 and 2.776,respectively.The repeatability was good under the different experimental conditions.There were no significant differences between the calculated values in QAMS and the estimated values in the external standard method by t-tests(P>0.05).Conclusion:The established QAMS method can be used for determining the contents of the five index components in Rupixiao tablets.

关 键 词：一测多评法 乳癖消片 连翘苷 芍药苷 三七皂苷R1 人参皂苷Rg1 人参皂苷RB1 

分 类 号：R927.2[医药卫生—药学]