氯化锰染毒对大鼠肝脏亚细胞器微量元素含量的影响  

Effects of manganese chloride on the trace element content of liver suborganelles in rats

作　　者：甄亚平 陈春霞[2] 敬海明[2,3] 黄沛力[2] 李国君[2,3] ZHEN Ya-ping;CHEN Chun-xia;JING Hai-ming;HUANG Pei-li;LI Guo-jun(Clinical Detection Center,Capital Medical University,Beijing 100069,China;不详)

机构地区：[1]首都医科大学临床检测中心,北京100069 [2]首都医科大学公共卫生与家庭医学学院卫生毒理学与卫生化学系,北京100069 [3]北京市疾病预防控制中心北京市预防医学研究中心食物中毒诊断溯源技术北京市重点实验室,北京100013

出　　处：《中国卫生检验杂志》2020年第1期1-3,8,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81373028)

摘　　要：目的探讨氯化锰(MnCl2)染毒对大鼠肝细胞核、线粒体、细胞膜微量元素的影响。方法雄性SD大鼠随机分为6组(30 d染毒组,90 d染毒组,90 d+30 d染毒组,30 d对照组,90 d对照组,90 d+30 d对照组),30 d染毒组和90 d染毒组分别经腹腔连续注射MnCl26 mg/(kg·d)(以Mn计)30 d、90 d(周日除外);90 d+30 d染毒连续注射MnCl290 d后停止染毒,继续正常喂养30 d。使用电感耦合等离子体-质谱(ICP-MS)仪测量大鼠肝细胞核、线粒体、细胞膜中Mn、Ca、Cu、Mg、Fe、Zn的含量。结果细胞核和细胞膜中的微量元素在染毒90 d后分别有不同程度的升高,线粒体中的微量元素除Mn和Ca元素含量显著升高外,均显著降低。结论肝细胞线粒体是Mn蓄积的主要场所,Mn对大鼠肝脏的损伤作用主要表现在对线粒体的损伤作用,染Mn后线粒体Cu、Fe、Zn、Mg含量降低,可能是Mn中毒引起肝线粒体损害的机制之一。Objective To investigate the effects of manganese chloride(MnCl2)on the trace elements in rat liver nuclei,mitochondria and cell membranes.Methods The male SD rats were intraperitoneally(ip)given MnCl26 mg(Mn)/kg·bw for 30 d,90 d,or 90 d followed by 30 d recovery.The samples of liver were collected at each time point.The contents of metals(Mn,Ca,Cu,Mg,Fe,Zn)in cellular nuclei,mitochondria and cell membranes of rat liver were measured by inductively coupled plasma-mass spectrometry(ICP-MS).Results The trace elements in nucleus and cell membrane increased after 90 days of exposure of MnCl2.The elements(Cu,Mg,Fe,Zn)in mitochondria decreased significantly,whereas Mn and Ca increased.Conclusion Hepatocyte mitochondria is the main site of Mn accumulation.The damage of Mn to the liver of rats is mainly the damage to mitochondria.The contents of Cu,Fe,Zn,and Mg in mitochondria decrease after Mn infection,which may be caused by Mn poisoning mechanism.iver injury.

关 键 词：二氯化锰 金属 肝脏 亚细胞器 电感耦合等离子体-质谱 

分 类 号：R114[医药卫生—卫生毒理学]