白细胞介素-32和胸腺基质淋巴细胞生成素在特应性皮炎患者外周血中的表达  

Expression of IL-32 and TSLP in peripheral blood of atopic dermatitis patients and their significance

作　　者：田兰馨 徐慧 彭柔 慈雪 王秀敏[1] TIAN lan-xin;XU Hui;PENG Rou(Binzhou Medical University Hospital,Binzhou 256600,China)

机构地区：[1]滨州医学院附属医院皮肤科,滨州256600 [2]聊城市东昌府区妇幼保健院皮肤科

出　　处：《实用皮肤病学杂志》2019年第6期344-347,共4页Journal of Practical Dermatology

摘　　要：目的探讨特应性皮炎(atopic dermatitis,AD)患者治疗前及治疗8周后外周血白细胞介素(IL)-32、胸腺基质淋巴细胞生成素(TSLP)的表达及意义。方法选取AD患者48例,健康对照组30例,采用酶联免疫吸附试验(ELISA)分别检测治疗前及治疗后外周血IL-32、TSLP的表达水平,分析治疗前后二者的表达意义及与AD病情严重程度(SCORAD)评分的相关性。结果AD患者治疗前IL-32、TSLP水平较正常对照组均显著升高(P均<0.01),且TSLP与SCORAD评分呈正相关(P<0.01)。AD患者应用他克莫司软膏联合复方甘草酸苷治疗后血清IL-32表达水平较治疗前明显降低(P<0.01),与正常对照组比差异无统计学意义(P>0.05);治疗后TSLP表达水平较治疗前明显降低(P<0.01),较正常对照组升高(P<0.01)。AD患者治疗前血清IL-32与TSLP水平之间有相关性,为正相关(P<0.01),治疗前SCORAD评分明显高于治疗后,差异具有统计学意义(P<0.01)。结论IL-32、TSLP在AD患者外周血中表达升高,并与病情严重程度呈正相关。应用他克莫司和复方甘草酸苷治疗可降低IL-32和TSLP在患者血清中的表达水平。Objective To explore the expression and significance of interleukin(IL)-32 and thymic stromal lymphopoietin(TSLP)in patients with atopic dermatitis(AD)before and after 8 weeks of treatment.Methods Forty-eight patients with atopic dermatitis and 30 healthy controls were selected,and the expression levels of IL-32 and TSLP in peripheral blood before and after treatment were detected by ELISA.The relationship between the expression level of IL-32 and TSLP and the severity of the disease(SCORAD score)was analyzed.Results IL-32 and TSLP levels in patients with AD before treatment were significantly higher than those in the normal control group(all P<0.01),and TSLP levels was positively correlated with SCORAD score(P<0.01).After the treatment of tacrolimus ointment combined with compound glycyrrhizin capsules,the expression level of serum IL-32 in patients with AD was significantly lower that of the treatment,but to be compared with the normal control group,there was no statistically significant difference(P>0.05).The expression level of TSLP after treatment was significantly lower than that of before the treatment(P<0.01),which was higher than that of the normal control group(P<0.01).There was a positive correlation between serum IL-32 and TSLP before treatment(P<0.01)in patients with AD.SCORAD score before treatment was significantly higher than that of after the treatment,and the difference was statistically significant(P<0.01).Conclusion The expression of IL-32 and TSLP in peripheral blood of AD patients was increased and positively correlated with the severity of the disease.Treatment with tacrolimus and compound glycyrrhizin reduced the expression levels of IL-32 and TSLP in patients’serum.

关 键 词：皮炎 特应性 白细胞介素-32 胸腺基质淋巴细胞生成素 他克莫司 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]