内蒙古鄂尔多斯地区献血者人群布氏菌感染情况调查  

Prevalence of Brucella spp. infection in blood donor population in Ordos area, Inner Mongolia, China

作　　者：陆锦辉 朱晓平 王佳雯 沈木生 司晋鸿 张玲 李婷婷 王文敬 付涌水[3] 黎诚耀 LU Jinhui;ZHU Xiaoping;WANG Jiawen;SHEN Musheng;SI Jinhong;ZHANG Ling;LI Tingting;WANG Wenjing;FU Yongshui;LI Chengyao(Department of Transfusion Medicine,Southern Medical University,Guangzhou 510515,China;Ordos Blood Center;Guangzhou Blood Center)

机构地区：[1]南方医科大学检验与生物技术学院输血医学系,广东广州510515 [2]鄂尔多斯中心血站 [3]广州血液中心

出　　处：《中国输血杂志》2020年第1期42-45,共4页Chinese Journal of Blood Transfusion

基　　金：广东省引进创新创业团队项目(2014Z05S123);国家卫健委委托项目:建立经血传播疾病病原体筛查技术方法

摘　　要：目的通过对内蒙古额鄂尔多斯地区矿区团体和街头的2个献血者人群,进行布鲁氏菌(简称"布菌")感染筛查,评估布菌对血液安全构成的潜在风险。方法采用侧向流动免疫分析(LFIA)、虎红平板试验(RBPT)、试管凝集试验(SAT)对献血者血浆中的布氏菌抗体进行初筛;随访可疑阳性标本,进行布菌分离培养,并对可疑阳性血液标本再进行ELISA,Western Blot及PCR检测。结果 2018年7月21日-8月23日期间,共检测鄂尔多斯地区献血者血浆标本1255份,其中矿区团体献血者837份,街头献血者418份。结果显示,共计17份血清标本出现至少1种或2种以上方法的布菌抗体检测阳性,占总数的1.35%。经ELISA和Western Blot鉴定,其中14份血清标本为抗体阳性,占总数1.12%(14/1255),其中矿区抗体阳性率为0.96%(8/837),街头抗体阳性率1.44%(6/418);3份血清标本呈Nested PCR阳性,阳性率为0.24%(3/1255)。结论在布鲁氏菌病流行的地区,感染发生在非职业风险人群中。布鲁氏菌感染的特征是细胞内寄生和持续性菌血症。鄂尔多斯地区献血者人群的布菌抗体阳性率为1.35%,核酸阳性率为0.24%,对血液安全性提出了警示。Objective In this study,we screened for Brucella spp. infection in two blood donor groups in the mining area and the streets of Ordos, Inner Mongolia, to assess the potential risk of Brucella to blood safety.Methods Lateral flow immunoassays(LFIA), Rose Bengal plate test(RBPT) and tube agglutination test(SAT) were applied to sift anti-Brucella antibodies in the plasma of blood donors preliminarily. The material of plasma with suspected positive Brucella infection was sampled for bacterial culture and isolation. The blood samples were eventually identified by methods of ELISA, Western-blot and Nest-PCR.Results During the period between July 21 st and August 23 rd in 2018, altogether 1255 blood samples from the Ordos region were detected. There were 837 blood donors from mining areas and the others from the streets. The results indicated that 17 plasma samples showed positive detection of brucellosis via one or more methods, which accounted for 1.35% of the total amount. 14 blood samples were tested positive by both ELISA and Western-blot, constituting 1.12%(14/1255) of overall samples. The positive rate of the mining area′s samples was 0.96%(8/837), while the one among the streets was 1.44%(6/418). The number of positive samples detected by Nest-PCR was 3, with a proportion of 0.24%(3/1255).Conclusion In areas where brucellosis is endemic, infections occur in non-occupational risk group. The characteristics of Brucella infection are intracellular parasitism and persistent bacteremia. The blood donors in the Ordos were with a 1.35% serological positive rate of Brucella, and the positive rate of the nucleic acid test was 0.32%, which could offer some warnings for blood safety.

关 键 词：布氏菌 流行率 献血者 鄂尔多斯地区 

分 类 号：R446.6[医药卫生—诊断学] Q939.1[医药卫生—临床医学]