酶联免疫吸附法与甲苯胺红不加热血清试验法在梅毒检验中的应用效果  

Application of ELISA Test and Toluidine Red Unheated Serum Test in Syphilis Detection

作　　者：李爱丽 黄丽芳 刘昊虹 LI Aili;HUANG Lifang;LIU Haohong(Department of Laboratory,First People's Hospital of Shaoguan,Shaoguan 512000,Guangdong Province,China)

机构地区：[1]韶关市第一人民医院检验科,广东韶关512000

出　　处：《中国实用乡村医生杂志》2020年第2期36-38,共3页Chinese Practical Journal of Rural Doctor

摘　　要：目的探讨酶联免疫吸附(ELISA)法与甲苯胺红不加热血清试验(TRUST)法在梅毒检测中的应用价值。方法选取2017年2月-2019年2月广东省韶关市第一人民医院收治的疑似梅毒患者320例为研究对象,所有患者均接受ELISA法与TRUST法检验,最终以梅毒螺旋体明胶颗粒凝集试验(TPPA)作为诊断的金标准,比较不同检验方法的阳性检出率及诊断效能。结果ELISA法在梅毒检验中的敏感性为96.19%,特异性为95.45%,准确性为95.94%,阳性预测值为97.58%;TRUST法在梅毒检验中的敏感性为79.52%,特异性为83.64%,准确性为80.94%,阳性预测值为90.27%;两组比较差异有统计学意义(P<0.05)。结论与TRUST法相比,ELISA法的阳性检出率及诊断效能更高,与TPPA检验结果相当,值得在梅毒检验中应用推广。Objective To explore the value of ELISA and TRUST in syphilis detection.Methods A total of 320 suspected syphilis patients were selected from February 2017 to February 2019 in our hospital.All patients were tested by ELISA and TRUST methods,and finally the treponema pallidum particle agglutination(TPPA)was used as the gold standard for diagnosis.The positive rates and diagnostic performances of different methods were compared.Results The sensitivity of ELISA in syphilis test was 96.19%,the specificity was 95.45%,the accuracy was 95.94%,the positive prediction value was 97.58%,the sensitivity of TRUST in syphilis test was 79.52%,the specificity was 83.64%,the accuracy was 80.94%,and the positive prediction value was 90.27%.There was a statistical difference between the two groups(P<0.05).Conclusion Compared with TRUST method,ELISA method has higher positive detection rate and diagnostic efficacy,and is comparable to TPPA results.It is worth applying in syphilis detection.

关 键 词：ELISA法 TRUST法 TPPA法 梅毒 阳性检出率 诊断效能 

分 类 号：R446[医药卫生—诊断学]