海南甘薯主栽品种再生体系的优化  

Optimization of Regeneration System of Main Sweet Patato Cultivars in Hainan,China

作　　者：孙言博 祝志欣 黄婷 李思明 朱国鹏[1] SUN Yanbo;ZHU Zhixin;HUANG Ting;LI Siming;ZHU Guopeng(School of Horticulture,Hainan University,Haikou,Hainan 570228,China;School of Life and Pharmaceutical Sciences,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学园艺学院,海南海口570228 [2]海南大学生命科学与药学院,海南海口570228

出　　处：《热带作物学报》2020年第2期244-251,共8页Chinese Journal of Tropical Crops

基　　金：海南省重点研发项目“海南甘薯主要病毒鉴定及主栽品种高效脱毒技术体系研究”(No.ZDYF2019030)。

摘　　要：本研究以海南甘薯主栽品种‘心香’、‘广薯87’、‘川山紫’、‘宁紫薯1号’、‘薯绿一号’、‘高系14’和‘三角宁’为材料,从外植体的选择、不同消毒方案、培养基配方和防褐化剂筛选等方面进行研究,建立一套适合热带地区甘薯主栽品种的脱毒快繁优化再生体系。结果表明:侧芽增殖率与存活率最高;在不同的外植体消毒处理中,依次用75%酒精消毒60 s,2%NaClO消毒15 min,以及0.1%HgCl2消毒15 min的污染率最低;最佳的生根培养基配方为:MS+0.05 mg/L NAA+0.1 mg/L GA3;茎长生长最快的培养基配方为:MS+0.1 mg/L NAA+1 mg/L 6-BA+0.5 mg/L GA3;干重增长最快的培养基配方为:MS+0.1 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA3;在培养基中加入5~6 g/L硫代硫酸钠和1.25 g/L聚乙烯吡咯烷酮能有效地抑制外植体的褐变。In this study,the main sweet potato varieties planted in tropical areas,including‘Xinxiang’,‘Guangshu 87’,‘Chuanshan Zi’,‘Ning Zishu 1’,‘Shulv 1’,‘Gaoxi 14’and‘Sanjiao Ning’were used as the materials.The selection of explants,disinfection schemes,medium formulation and anti-browning agent were explored.The optimum regeneration system of virus-free and rapid propagation was established for the main sweet potato varieties.The proliferation rate and survival rate of lateral buds were the highest among different parts of explants.In treatments of different explant disinfection,75%alcohol for 60 s,2%sodium hypochlorite for 15 min,and 0.1%mercury chloride for 15 min exhibited the lowest contamination rate.The medium formula with the shortest rooting time was as follows:MS+0.05 mg/L NAA+0.1 mg/L GA3.The medium formula with the fastest growth of stem length was as follows:MS+0.1 mg/L NAA+1 mg/L 6-BA+0.5 mg/L GA3.The medium formula with the fastest growth rate of dry weight was as follows:MS+0.1 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA3.Adding 5–6 g/L sodium thiosulfate and 1.25 g/L polyvinylpyrrolidone to the medium could effectively inhibit the browning of explants.

关 键 词：甘薯 再生体系 培养基配方 外植体褐变 

分 类 号：S531[农业科学—作物学]