花粉管通道法转化荔枝的初步研究  

Gene Transformation of Litchi via Pollen Tube Pathway

作　　者：王树军[1] 孙进华[1] 李焕苓[1] 王果[1] 李芳 王家保[1] WANG Shujun;SUN Jinhua;LI Huanling;WANG Guo;LI Fang;WANG Jiabao(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《热带作物学报》2020年第2期252-257,共6页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.319QN270);国家现代农业产业技术体系(No.CARS-32-03)。

摘　　要：为开拓荔枝转基因育种新途径,以GUS基因作为报告基因,用花粉管通道法转化授粉后24 h的‘新球蜜荔’荔枝雌花,并统计座果率、成苗率、转化率。结果显示:共获得303株实生苗,经PCR检测和GUS染色法证实外源基因整合到4株荔枝苗基因组中,转化率为1.32%。此研究结果为荔枝生物技术育种提供了一种简单有效的途径。In order to develop a new gene transformation pathway for litchi,the female flowers of Xinqiumili(24 h after pollination)were transformed by the pollen tube pathway method,using GUS as a report gene,and the fruit set percentage,seeding rate,and conversion rate were counted.A total number of 303 seedlings were obtained,and four positive transformed plants were verified according to PCR analysis and GUS staining,the transformation rate was 1.32%.This study would provide a simple and efficient way for the biotechnology breeding of litchi.

关 键 词：荔枝 花粉管通道 转基因 育种 

分 类 号：S667.1[农业科学—果树学]